Please use this identifier to cite or link to this item: https://biore.bio.bg.ac.rs/handle/123456789/325
Title: Microscopy investigation of glandular trichomes of Satureja horvatii Šilić (Lamiaceae)
Authors: Marin, Marija 
Ascensao, Lia
Jasnić, Nebojša 
Branković, S.
Keywords: Microscopy;Glandular trichomes;Satureja horvatii Šilić
Issue Date: 25-Aug-2013
Rank: M33
Conference: Microscopy Conference
Abstract: 
The essential oils isolated from various Satureja species, which belong to the family Lamiaceae,
have been shown to posses biological and pharmacological activities, such as antibacterial, antifungal,
antiviral, antioxidant [1,2]. Satureja horvatii Šilić is an endemic species [3] of the Orjen-Lovćen
mountain massif in Montenegro with a high content of the essential oil.
Considering the importance of Satureja horvatii Šilić as an endemic plant, and due to the essential oils
produced in its glandular trichomes, we carried out micromorphological and ultrastructural analyses of
plant’s glandular trichomes as a primary secretory organs and have also analysed autofluorescense of
their secreted products.
The investigation was carried out using scanning electron (JEOL JSM T 220 15 kV), transmission
electron (Philips TEM 208 S.), confocal laser scanning (CLSM 510 Carl Zeiss with Axioskop FS2 mot
), and light fluorescence (Leica DM LS with a 13 BLU 450-490 nm filter) microscopy.
A leaf cross-section of Satureja horvatii indicated the presence of several different morphological
types of glandular and non-glandular trichomes Figure 1. The adaxial and abaxial leaf sides of
Satureja horvatii were covered by an indumentum containing peltate, capitate and digitiform trichomes,
as well as unicellular and multicellular unbranched non-glandular trichomes with wart-like cuticular
structures Figure 2. Peltate trichomes distributed on the adaxial and abaxial leaf sides consisted of
one basal epidermal cell, a wide stalk cell and a multicellular head consisting of twelve cells. Capitate
trichomes, uniformly distributed on both leaf surfaces, were divided into two types according to the
shape of the unicellular secretory head. Type I was composed of one basal cell, one stalk cell and
spherical unicellular head, while type II was composed of one basal cell, one stalk cell and an ellipsoid
unicellular head. Capitate trichomes were more numerous than peltate and digitiform trichomes on the
both leaf sides. Digitiform trichomes, which do not show a clear distinction between the apical
glandular cell and the subsidiary cells, occurred between the peltate and capitate trichomes with a
smaller distribution. Non-glandular trichomes were densely distributed on the adaxial and abaxial leaf
sides and on the margins of the leaves. Ultrastructural analyses showed the presence of numerous
lipid globules and proliferations of the granular endoplasmatic reticulum in secretory phases of the
heads of glandular trichomes. The outer cell wall was covered with a thick cuticle Figure 3.
Strong red autofluorescence of the lipophilic and hydrophylic secreted material was observed with
CLSM Figure 4. While intensive light yellow autofluorescence was noticed in peltate trichomes using
fluorescence microscope Figure 5. With the increasing tendency to use volatile oils, our investigation, together with the results of chemical investigations, indicate that S. horvatii essential oil could be applied in the pharmaceutical industries.
URI: https://biore.bio.bg.ac.rs/handle/123456789/325
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