Please use this identifier to cite or link to this item: https://biore.bio.bg.ac.rs/handle/123456789/260
Title: Short-term fasting increases the levels of glucose transporters in the male rat hypothalamus
Authors: Dakić, Tamara 
Jevđović, Tanja 
Lakić, Iva 
Perić, Mina 
Jasnić, Nebojša 
Đorđević, Jelena 
Vujović, Predrag 
Keywords: Short-term fasting;Glucose transporters;Hypothalamus
Issue Date: 25-Oct-2017
Publisher: Serbian Neuroscience Society
Conference: 7th Congress of Serbian Neuroscience Society
Abstract: 
Introduction. Different isoforms of glucose transporters (GLUTs) are present in
the brain and they exhibit cell-specific expression and distinct anatomical
localization. GLUT1 and GLUT3 are the predominant types of glucose
transporters in the brain, whereas other forms are less abundant and are
involved in glucose sensing only in certain brain regions. Here we examined the
effect of six-hour fasting on the levels and anatomical distribution of GLUT1,
GLUT2, and GLUT3 in the rat hypothalamus. Methods. Food was removed at 6
pm and male Wistar rats were sacrificed at midnight. Control animals had ad
libitum access to food and they were sacrificed simultaneously with the fasting
rats. The hypothalamic membrane protein fraction was isolated using
subcellular protein fractionation kit for tissues. The amount of GLUT1, 2, and 3
was estimated by immunoblotting. Immunofluorescence was used to determine
cell types expressing each of the examined GLUTs as well as their anatomical
localizations within the hypothalamus. Results. Short-term food deprivation
increased content of all three examined GLUT isoforms in the hypothalamic
membrane protein fraction. GLUT1 and GLUT2 immunopositivity was observed
in neuronal cell bodies and in the ependymal cells surrounding the third
ventricle. GLUT1 was detected throughout the entire hypothalamic region, while
strong GLUT2 immunoreactivity was restricted to paraventricular and
ventromedial nucleus. GLUT3 positivity was detected in the subventricular zone
glial cells located in the vicinity of ependymal cells. Conclusion. In conclusion,
although the short-term fasting increased the hypothalamic levels of the
examined GLUTs, it did not result in changing their regional distribution in this
brain region.
URI: https://biore.bio.bg.ac.rs/handle/123456789/260
ISBN: 978-86-917255-1-8
Appears in Collections:Conference abstract

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