Please use this identifier to cite or link to this item: https://biore.bio.bg.ac.rs/handle/123456789/248
Title: Effects of a high-fat diet on expression of the hypothalamic NADPH oxidases
Authors: Ružičić, Aleksandra 
Jasnić, Nebojša 
Jevđović, Tanja 
Dakić, Tamara 
Klop Mathieu
Kračun, Damir
Đorđević, Jelena 
Keywords: high-fat diet;NADPH oxidases;Hypothalamus
Issue Date: 9-May-2019
Publisher: Farmaceutski fakultet - Univerzitet u Beogradu
Conference: 2nd Symposium in Biomedicine: Basic and Clinical Neuroscience
Abstract: 
Background and Aims - Obesity is an increasing epidemic worldwide; therefore, numerous clinical and basic studies focus on the potential mechanisms underlying this complex disorder. High-fat (HF) diet-induced obesity is associated with mild inflammation in peripheral tissues. NADPH oxidases (NOX) are recognized as a physiological source of reactive oxygen species (ROS) contributing to the inflammation. Previous studies proposed that similar events may also occur in the hypothalamus, a cerebral area responsible for the regulation of energy homeostasis.
Purpose of this study was to examine the effects of a HF diet on expression of the hypothalamic NADPH oxidases. We also examined expression of transcription factor HIF-1alpha (HIF-1α) owing to its potential involvement in the upregulation of the NOX system.
Methods - Male rats were placed on HF diet starting at 9 weeks of age and lasting for 12 weeks. Expression of NOX 2/gp91phox, NOX 4, p22phox subunit, and HIF-1α in the hypothalamus was evaluated by Western blot analysis.
Results - The HF diet significantly increased the expression of NOX 4 and its p22phox subunit in the hypothalamus. No significant changes in the levels of NOX 2 and HIF-1α were observed.
Conclusions – The present study suggests that NOX 4 but not NOX 2, might be involved in the onset of the hypothalamic inflammation underlying the HF diet-induced obesity. The involvement of HIF-1α needs to be further investigated.
URI: https://biore.bio.bg.ac.rs/handle/123456789/248
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