Please use this identifier to cite or link to this item: http://biore.bio.bg.ac.rs/handle/123456789/1064
Title: Brain cortical injury induces changes in peripheral lymphocyte ectonucleotidase activities
Authors: Laketa, Danijela 
Bjelobaba, Ivana
Stojkov, Danijela
Lavrnja, Irena
Parabucki, Ana
Stojiljković, Mirjana
Nedeljković, Nadežda 
Keywords: Adenosine;ADP;ATP;E-NTPDase;Ecto-5'-nucleotidase brain injury
Issue Date: 6-Feb-2013
Journal: Archives of Biological Sciences
Abstract: 
Injury and other pathological conditions induce a massive release of ATP and ADP that initiate an immune response. Extracellular nucleotides are degraded by ectonucleotidases: enzymes from E-NTPDase and E-NPP families sequentially hydrolyze ATP and ADP to AMP, which is further hydrolyzed by ecto-5'-nucleotidase to adenosine that exerts suppressive effects on immune cells. We investigated the ectonucleotidase activities of peripheral lymphocytes at different post-injury times after an unilateral brain injury in the rat. Significant and dynamic changes in the lymphocytic ectonucleotidase activities were obtained. ATP- and ADP-hydrolysis changes, together with their calculated ratios, indicate the major contribution of E-NTPDase 1 and its comparable upregulation between sham operation and injury. AMP hydrolysis changes were more brain-injury specific, with a longer-lasting lymphocytic response induced by cortical stab injury (CSI). In summary, CSI and sham operation induce the upregulation of the whole enzyme chain for adenine nucleotide hydrolysis in lymphocytes, suggesting an important roles of ectonucleotidases in the course of recovery after brain injury.
URI: http://hdl.handle.net/123456789/1064
ISSN: 03544664
DOI: 10.2298/ABS1301033L
Appears in Collections:Journal Article

Files in This Item:
File Description SizeFormat Existing users please Login
27 BRAIN CORTICAL INJURY INDUCES CHANGES IN PERIPHERAL LYMPHOCYTE .pdf488.93 kBAdobe PDF    Request a copy
Show full item record

SCOPUSTM   
Citations

2
checked on Sep 24, 2021

Page view(s)

5
checked on Feb 7, 2020

Google ScholarTM

Check

Altmetric

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.