Please use this identifier to cite or link to this item: https://biore.bio.bg.ac.rs/handle/123456789/992
Title: Nitric oxide regulates mitochondrial re-modelling in interscapular brown adipose tissue: Ultrastructural and morphometric-stereologic studies
Authors: Petrović, V.
Korać, Aleksandra 
Buzadžić, B.
Vasilijević, A.
Janković, A.
Veličković, Ksenija 
Korać, Bato 
Keywords: Brown adipocytes;Electron microscopy;Mitochondriogenesis;Nitric oxide
Issue Date: 1-Dec-2008
Project: This work was supported by the Ministry of Science, Republic of Serbia, grant no. 143050
COST FA0602 Action
Journal: Journal of Microscopy
Abstract: 
As a complex, cell-specific process that includes both division and clear functional differentiation of mitochondria, mitochondriogenesis is regulated by numerous endocrine and autocrine factors. In the present ultrastructural study, in vivo effects of l-arginine-nitric oxide (NO)-producing pathway on mitochondriogenesis in interscapular brown adipose tissue (IBAT) were examined. For that purpose, adult Mill Hill hybrid hooded rats were receiving l-arginine, a substrate of NO synthases (NOSs), or Nω-nitro-l-arginine methyl ester (l-NAME), an inhibitor of NOSs, as drinking liquids for 45 days. All experimental groups were divided into two sub-groups - acclimated to room temperature and cold. IBAT mitochondria were analyzed by transmission electron microscopy and stereology. l-Arginine treatment acted increasing the number of mitochondrial profiles per cell profile, as well as volume fraction of mitochondria per cell volume in animals maintained at room temperature. Cold-induced enhancement of number of mitochondrial profiles per cell profile was additionally increased in l-arginine-treated rats. Ultrastructural examinations of l-arginine-treated cold-acclimated animals clearly demonstrated thermogenically active mitochondria (larger size, lamellar, more numerous and well-ordered cristae in their profiles), which however were inactive in l-arginine-receiving animals kept at room temperature (small mitochondria, tubular cristae). By contrast, l-NAME treatment of rats acclimated to room temperature induced mitochondrial alterations characterized by irregular shape, short disorganized cristae and megamitochondria formation. These results showed that NO is a necessary factor for mitochondrial biogenesis and that it acts intensifying this process, but NO alone is not a sufficient stimulus for in vivo induction of mitochondriogenesis in brown adipocytes. © 2008 The Authors.
URI: https://biore.bio.bg.ac.rs/handle/123456789/992
ISSN: 0022-2720
DOI: 10.1111/j.1365-2818.2008.02132.x
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