Please use this identifier to cite or link to this item:
https://biore.bio.bg.ac.rs/handle/123456789/992
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Petrović, V. | en_US |
dc.contributor.author | Korać, Aleksandra | en_US |
dc.contributor.author | Buzadžić, B. | en_US |
dc.contributor.author | Vasilijević, A. | en_US |
dc.contributor.author | Janković, A. | en_US |
dc.contributor.author | Veličković, Ksenija | en_US |
dc.contributor.author | Korać, Bato | en_US |
dc.date.accessioned | 2019-07-22T08:39:43Z | - |
dc.date.available | 2019-07-22T08:39:43Z | - |
dc.date.issued | 2008-12-01 | - |
dc.identifier.issn | 0022-2720 | - |
dc.identifier.uri | https://biore.bio.bg.ac.rs/handle/123456789/992 | - |
dc.description.abstract | As a complex, cell-specific process that includes both division and clear functional differentiation of mitochondria, mitochondriogenesis is regulated by numerous endocrine and autocrine factors. In the present ultrastructural study, in vivo effects of l-arginine-nitric oxide (NO)-producing pathway on mitochondriogenesis in interscapular brown adipose tissue (IBAT) were examined. For that purpose, adult Mill Hill hybrid hooded rats were receiving l-arginine, a substrate of NO synthases (NOSs), or Nω-nitro-l-arginine methyl ester (l-NAME), an inhibitor of NOSs, as drinking liquids for 45 days. All experimental groups were divided into two sub-groups - acclimated to room temperature and cold. IBAT mitochondria were analyzed by transmission electron microscopy and stereology. l-Arginine treatment acted increasing the number of mitochondrial profiles per cell profile, as well as volume fraction of mitochondria per cell volume in animals maintained at room temperature. Cold-induced enhancement of number of mitochondrial profiles per cell profile was additionally increased in l-arginine-treated rats. Ultrastructural examinations of l-arginine-treated cold-acclimated animals clearly demonstrated thermogenically active mitochondria (larger size, lamellar, more numerous and well-ordered cristae in their profiles), which however were inactive in l-arginine-receiving animals kept at room temperature (small mitochondria, tubular cristae). By contrast, l-NAME treatment of rats acclimated to room temperature induced mitochondrial alterations characterized by irregular shape, short disorganized cristae and megamitochondria formation. These results showed that NO is a necessary factor for mitochondrial biogenesis and that it acts intensifying this process, but NO alone is not a sufficient stimulus for in vivo induction of mitochondriogenesis in brown adipocytes. © 2008 The Authors. | en_US |
dc.language.iso | en | en_US |
dc.relation | This work was supported by the Ministry of Science, Republic of Serbia, grant no. 143050 | en_US |
dc.relation | COST FA0602 Action | en_US |
dc.relation.ispartof | Journal of Microscopy | en_US |
dc.subject | Brown adipocytes | en_US |
dc.subject | Electron microscopy | en_US |
dc.subject | Mitochondriogenesis | en_US |
dc.subject | Nitric oxide | en_US |
dc.title | Nitric oxide regulates mitochondrial re-modelling in interscapular brown adipose tissue: Ultrastructural and morphometric-stereologic studies | en_US |
dc.type | Article | en_US |
dc.identifier.doi | 10.1111/j.1365-2818.2008.02132.x | - |
dc.identifier.pmid | 19094038 | - |
dc.identifier.scopus | 2-s2.0-57449099876 | - |
dc.identifier.url | https://api.elsevier.com/content/abstract/scopus_id/57449099876 | - |
item.languageiso639-1 | en | - |
item.cerifentitytype | Publications | - |
item.openairetype | Article | - |
item.fulltext | No Fulltext | - |
item.grantfulltext | none | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
crisitem.author.dept | Chair of Cell and Tissue Biology | - |
crisitem.author.dept | Chair of Cell and Tissue Biology | - |
crisitem.author.orcid | 0000-0002-3044-9963 | - |
crisitem.author.orcid | 0000-0002-4373-5483 | - |
crisitem.author.orcid | 0000-0001-5272-579X | - |
Appears in Collections: | Journal Article |
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