Please use this identifier to cite or link to this item: https://biore.bio.bg.ac.rs/handle/123456789/992
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dc.contributor.authorPetrović, V.en_US
dc.contributor.authorKorać, Aleksandraen_US
dc.contributor.authorBuzadžić, B.en_US
dc.contributor.authorVasilijević, A.en_US
dc.contributor.authorJanković, A.en_US
dc.contributor.authorVeličković, Ksenijaen_US
dc.contributor.authorKorać, Batoen_US
dc.date.accessioned2019-07-22T08:39:43Z-
dc.date.available2019-07-22T08:39:43Z-
dc.date.issued2008-12-01-
dc.identifier.issn0022-2720-
dc.identifier.urihttps://biore.bio.bg.ac.rs/handle/123456789/992-
dc.description.abstractAs a complex, cell-specific process that includes both division and clear functional differentiation of mitochondria, mitochondriogenesis is regulated by numerous endocrine and autocrine factors. In the present ultrastructural study, in vivo effects of l-arginine-nitric oxide (NO)-producing pathway on mitochondriogenesis in interscapular brown adipose tissue (IBAT) were examined. For that purpose, adult Mill Hill hybrid hooded rats were receiving l-arginine, a substrate of NO synthases (NOSs), or Nω-nitro-l-arginine methyl ester (l-NAME), an inhibitor of NOSs, as drinking liquids for 45 days. All experimental groups were divided into two sub-groups - acclimated to room temperature and cold. IBAT mitochondria were analyzed by transmission electron microscopy and stereology. l-Arginine treatment acted increasing the number of mitochondrial profiles per cell profile, as well as volume fraction of mitochondria per cell volume in animals maintained at room temperature. Cold-induced enhancement of number of mitochondrial profiles per cell profile was additionally increased in l-arginine-treated rats. Ultrastructural examinations of l-arginine-treated cold-acclimated animals clearly demonstrated thermogenically active mitochondria (larger size, lamellar, more numerous and well-ordered cristae in their profiles), which however were inactive in l-arginine-receiving animals kept at room temperature (small mitochondria, tubular cristae). By contrast, l-NAME treatment of rats acclimated to room temperature induced mitochondrial alterations characterized by irregular shape, short disorganized cristae and megamitochondria formation. These results showed that NO is a necessary factor for mitochondrial biogenesis and that it acts intensifying this process, but NO alone is not a sufficient stimulus for in vivo induction of mitochondriogenesis in brown adipocytes. © 2008 The Authors.en_US
dc.language.isoenen_US
dc.relationThis work was supported by the Ministry of Science, Republic of Serbia, grant no. 143050en_US
dc.relationCOST FA0602 Actionen_US
dc.relation.ispartofJournal of Microscopyen_US
dc.subjectBrown adipocytesen_US
dc.subjectElectron microscopyen_US
dc.subjectMitochondriogenesisen_US
dc.subjectNitric oxideen_US
dc.titleNitric oxide regulates mitochondrial re-modelling in interscapular brown adipose tissue: Ultrastructural and morphometric-stereologic studiesen_US
dc.typeArticleen_US
dc.identifier.doi10.1111/j.1365-2818.2008.02132.x-
dc.identifier.pmid19094038-
dc.identifier.scopus2-s2.0-57449099876-
dc.identifier.urlhttps://api.elsevier.com/content/abstract/scopus_id/57449099876-
item.languageiso639-1en-
item.cerifentitytypePublications-
item.openairetypeArticle-
item.fulltextNo Fulltext-
item.grantfulltextnone-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
crisitem.author.deptChair of Cell and Tissue Biology-
crisitem.author.deptChair of Cell and Tissue Biology-
crisitem.author.orcid0000-0002-3044-9963-
crisitem.author.orcid0000-0002-4373-5483-
crisitem.author.orcid0000-0001-5272-579X-
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