Please use this identifier to cite or link to this item: https://biore.bio.bg.ac.rs/handle/123456789/937
Title: Spatial and temporal patterns of oxidative stress in the brain of gerbils submitted to different duration of global cerebral ischemia
Authors: Selakovic, Vesna
Korenić, Andrej 
Radenović, Lidija 
Keywords: Brain;Gerbils;Nitric oxide;Reactive oxygen species;Reperfusion;Stroke
Issue Date: 1-Oct-2011
Journal: International Journal of Developmental Neuroscience
Abstract: 
The present study was undertaken to examine spatial and temporal patterns of oxidative stress rate in the brain of Mongolian gerbils submitted to different duration of global ischemia/reperfusion. The common carotid arteries of gerbils were occluded for 5, 10, or 15. min. We followed the temporal ischemia-induced oxidative stress rate, the most important factor that exacerbates brain damage by reperfusion, starting from 24. h up to 28 days after reperfusion. The spatial ischemia-induced oxidative stress distribution was measured parallely in different brain regions: forebrain cortex, striatum, hippocampus and cerebellum. Post-ischemic effects were followed in vivo by monitoring the neurological status of whole animals and at the intracellular level by standard biochemical assays in different brain regions. We measured superoxide production, superoxide dismutase activity, nitric oxide production, index of lipid peroxidation, and reduced glutathione. Our results revealed a pattern of dynamic changes in each oxidative stress parameter that corresponded with ischemia duration in all tested brain structures. The highest levels were obtained in the first 24. h after the insult. After that, they slowly returned to nearly control values 28 days after reperfusion (with the exception of SOD activity that returned to control values at fourth day after reperfusion). The most sensitive oxidative stress parameter was index of lipid peroxidation. Our study confirmed spatial distribution of ischemia-induced oxidative stress. Tested brain structures showed different sensitivity to each oxidative stress parameter, although their basal levels were similar. These new findings could be valuable for creation and strategy of post-ischemic therapy. © 2011 ISDN.
URI: https://biore.bio.bg.ac.rs/handle/123456789/937
ISSN: 0736-5748
DOI: 10.1016/j.ijdevneu.2011.02.009
Appears in Collections:Journal Article

Files in This Item:
Show full item record

SCOPUSTM   
Citations

33
checked on Oct 31, 2024

Page view(s)

3
checked on Nov 4, 2024

Google ScholarTM

Check

Altmetric

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.