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https://biore.bio.bg.ac.rs/handle/123456789/6928
Title: | Statistical analysis-based green planar chromatographic methodology for the quality assessment of food supplements: a case study on Origanum vulgare L. commercial products | Authors: | Jović, Marko Ristivojević, Petar Živković-Radovanović, Vukosava Andrić, Filip Dimkić, Ivica Milojković-Opsenica, Dušanka Trifković, Jelena |
Keywords: | Bioautography;;Commercial products;;Green planar chromatography;;Quality assessment;;Sum of ranking difference. | Issue Date: | 2023 | Rank: | M23 | Publisher: | SpringerLink | Journal: | JPC – Journal of Planar Chromatography – Modern TLC | Abstract: | Quality assessment is the most important step in the production of effective and safe food supplements. The aim of this study is to develop a green, simple, and high-throughput procedure for the quality assessment of Origanum vulgare L. commercial products using high-performance thin-layer chromatography (HPTLC)/bioautography with a multicriteria decision method, sum of ranking differences (SRD). Direct bioautography assays were developed for the identification of constituents with antibacterial activity tested against Gram-positive (Bacillus subtilis, Micrococcus lysodeikticus) and Gram-negative (Escherichia coli) strains, antioxidative (DPPH) activity, and enzyme (α-amylase) activity. Combination of the leave-one-out cross-validation methodology of SRD values and significant differences by post hoc Wilcoxon matched pairs test were used to evaluate commercial varieties. It was proved that suggested bioautography/chemometric screening methodology could be used as guidelines for the selection of any theoretical ideal product. Analysts may have benefits from this time-saving and streamlined quality profiling. |
URI: | https://biore.bio.bg.ac.rs/handle/123456789/6928 | ISSN: | 0933-4173 1789-0993 |
DOI: | 10.1007/s00764-023-00261-0 |
Appears in Collections: | Journal Article |
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