Please use this identifier to cite or link to this item: https://biore.bio.bg.ac.rs/handle/123456789/6809
Title: Rat brown adipose tissue thermogenic markers are modulated by estrous cycle phases and short-term fasting
Authors: Dakić, Tamara 
Veličković, Ksenija 
Lakić, Iva 
Ružičić, Aleksandra 
Milicevic, Andjela
Plackic, Nikola
Vujović, Predrag 
Jevđović, Tanja 
Keywords: brown adipose tissue;cellular plasticity;estrous cycle;short-term fasting;thermogenic markers
Issue Date: 21-Jul-2023
Rank: M21
Publisher: IUBMB Journals
Project: Ministry of Science, Technological Development and Innovation of the Republic of Serbia
Journal: BioFactors (Oxford, England)
Abstract: 
Brown adipose tissue (BAT) converts chemical energy into heat to maintain body temperature. Although fatty acids (FAs) represent a primary substrate for uncoupling protein 1 (UCP1)-dependent thermogenesis, BAT also utilizes glucose for the same purpose. Considering that estrous cycle effects on BAT are not greatly explored, we examined those of 6-h fasting on interscapular BAT (iBAT) thermogenic markers in proestrus and diestrus. We found that the percentage of multilocular adipocytes was lower in proestrus than in diestrus, although it was increased after fasting in both analyzed estrous cycle stages. Furthermore, the percentage of paucilocular adipocytes was increased by fasting, unlike the percentage of unilocular cells, which decreased in both analyzed stages of the estrous cycle. The UCP1 amount was lower in proestrus irrespectively of the examined dietary regimens. Regarding FA transporters, it was shown that iBAT CD36 content was increased in fasted rats in diestrus. In contrast to GLUT1, the level of GLUT4 was interactively modulated by selected estrous cycle phases and fasting. There was no change in insulin receptor and ERK1/2 activation, while AKT activation was interactively modulated by fasting and estrous cycle stages. Our study showed that iBAT exhibits morphological and functional changes in proestrus and diestrus. Moreover, iBAT undergoes additional dynamic functional and morphological changes during short-term fasting to modulate nutrient utilization and adjust energy expenditure.
URI: https://biore.bio.bg.ac.rs/handle/123456789/6809
ISSN: 09516433
DOI: 10.1002/biof.1993
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