Please use this identifier to cite or link to this item: https://biore.bio.bg.ac.rs/handle/123456789/6438
Title: Effects of maternal obesity on Wharton’s Jelly mesenchymal stromal cells
Authors: Badraiq, Heba
Čvoro, Aleksandra 
Galleu, Antonio
Simon, Marisa
Miere, Cristian
Hobbs, Carl
Schulz, Reiner
Siow, Richard
Dazzi, Francesco
Ilić, Duško
Issue Date: Dec-2017
Rank: M21
Publisher: NATURE PUBLISHING GROUP, LONDON, ENGLAND
Journal: Scientific Reports
Volume: 7
Issue: 1
Start page: 17595
Abstract: 
We investigated whether maternal metabolic environment affects mesenchymal stromal/stem cells (MSCs) from umbilical cord's Wharton's Jelly (WJ) on a molecular level, and potentially render them unsuitable for clinical use in multiple recipients. In this pilot study on umbilical cords post partum from healthy non-obese (BMI = 19-25; n = 7) and obese (BMI ≥ 30; n = 7) donors undergoing elective Cesarean section, we found that WJ MSC from obese donors showed slower population doubling and a stronger immunosuppressive activity. Genome-wide DNA methylation of triple positive (CD73+CD90+CD105+) WJ MSCs found 67 genes with at least one CpG site where the methylation difference was ≥0.2 in four or more obese donors. Only one gene, PNPLA7, demonstrated significant difference on methylome, transcriptome and protein level. Although the number of analysed donors is limited, our data suggest that the altered metabolic environment related to excessive body weight might bear consequences on the WJ MSCs.
URI: https://biore.bio.bg.ac.rs/handle/123456789/6438
DOI: 10.1038/s41598-017-18034-1
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