Please use this identifier to cite or link to this item: https://biore.bio.bg.ac.rs/handle/123456789/61
Title: Short-term fasting promotes insulin expression in rat hypothalamus
Authors: Dakić, Tamara 
Jevđović, Tanja 
Perić, Mina 
Bjelobaba, Ivana
Markelić, Milica 
Milutinović, Bojana
Lakić, Iva 
Jasnić, Nebojša 
Đorđević, Jelena 
Vujović, Predrag 
Keywords: fasting;glucose;hypothalamus;insulin;rat
Issue Date: 1-Jul-2017
Rank: M22
Journal: European Journal of Neuroscience
Abstract: 
In the hypothalamus, insulin takes on many roles involved in energy homoeostasis. Therefore, the aim of this study was to examine hypothalamic insulin expression during the initial phase of the metabolic response to fasting. Hypothalamic insulin content was assessed by both radioimmunoassay and Western blot. The relative expression of insulin mRNA was examined by qPCR. Immunofluorescence and immunohistochemistry were used to determine the distribution of insulin immunopositivity in the hypothalamus. After 6-h fasting, both glucose and insulin levels were decreased in serum but not in the cerebrospinal fluid. Our study showed for the first time that, while the concentration of circulating glucose and insulin decreased, both insulin mRNA expression and insulin content in the hypothalamic parenchyma were increased after short-term fasting. Increased insulin immunopositivity was detected specifically in the neurons of the hypothalamic periventricular nucleus and in the ependymal cells of fasting animals. These novel findings point to the complexity of mechanisms regulating insulin expression in the CNS in general and in the hypothalamus in particular.
URI: https://biore.bio.bg.ac.rs/handle/123456789/61
ISSN: 0953-816X
DOI: 10.1111/ejn.13607
Appears in Collections:Journal Article

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