Please use this identifier to cite or link to this item: https://biore.bio.bg.ac.rs/handle/123456789/60
Title: Morphofunctional parameters of rat somatotrophes after acute and repeated immobilization or restraint stress
Authors: Trifunović, Svetlana
Lakić, Iva 
Vujović, Predrag 
Jevđović, Tanja 
Šošić-Jurjević, Branka
Milošević, Verica
Đorđević, Jelena 
Keywords: Acute and repeated stress;Growth hormone;Histology;Pituitary;Somatotrophs;Stereology
Issue Date: 1-Jan-2019
Rank: M23
Journal: Acta Histochemica
Abstract: 
It is well known that stress changes levels of pituitary hormones in the bloodstream and in the pituitary itself. However, almost nothing is known about the impact of stress on histological and stereological parameters of the growth hormone producing cells (somatotrophs-GH cells). The aim of the present study was to investigate the effect of: acute and repeated immobilization; acute and repeated restraint on histological and morphofunctional parameters of somatotrophs in adult Wistar rats. Changes in the pituitary gland volume; the volume density and volume of somatotrophs following acute and repeated immobilization (IMO, R-IMO); acute and repeated restraint (R, R-R) were evaluated using a stereological system (newCAST), while growth hormone level within pituitary was determined by Western blot. Our results demonstrated the decrease (p < 0.05) of the pituitary volume (17%, 19%) in the IMO and R groups, respectively, and the increase in the R-R group. The volume density of GH cells decreased (p < 0.05) in the R-IMO (7%), R (26%) and R-R (18%) group in comparison to the control value. The pituitary GH content was increased (p < 0.05) after the IMO (2-fold), R (2.5-fold) and R-R (2.1-fold) as compared to the control group. These results point out that acute and repeated immobilization and/or restraint lead not only to changes in GH hormone concentration, but also modify the morphological aspects of GH cells within the rat pituitary.
URI: https://biore.bio.bg.ac.rs/handle/123456789/60
ISSN: 0065-1281
DOI: 10.1016/j.acthis.2018.10.003
Appears in Collections:Journal Article

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