Please use this identifier to cite or link to this item: https://biore.bio.bg.ac.rs/handle/123456789/5742
Title: Genotyping-driven diversity assessment of biocontrol potent Bacillus spp. strain collection as a potential method for the development of strain-specific biomarkers
Authors: Rosić, Iva 
Nikolić, Ivan 
Ranković, Tamara 
Anteljević, Marina 
Medić, Olja 
Berić, Tanja 
Stanković, Slaviša 
Keywords: Bacillus spp.;Biocontrol;Genotyping;Microbial diversity;RAPD;Rep
Issue Date: 13-Feb-2023
Rank: M23
Publisher: National Library of Medicine
Journal: Archives of Microbiology
Volume: 205
Issue: 4
Start page: 114
Abstract: 
Bacillus species are among the most researched and frequently applied biocontrol agents. To estimate their potential as environmentally friendly microbial-based products, reliable and rapid plant colonization monitoring methods are essential. We evaluated repetitive element-based (rep) and Random Amplified Polymorphic DNA (RAPD) PCR (Polymerase Chain Reaction) genotyping in a diversity assessment of 251 strains from bulk soil, straw, and manure samples across Serbia, highlighting their discriminative force and the presence of unique bands. RAPD 272, OPG 5, and (GTG)5 primers were most potent in revealing the high diversity of a sizable environmental Bacillus spp. collection. RAPD 272 also amplified a unique band for a proven biocontrol strain, opening the possibility of Sequence Characterized Amplified Region (SCAR) marker design. That will enable colonization studies using the SCAR marker for its specific detection. This study provides a guide for primer selection for diversity and monitoring studies of environmental Bacillus spp. isolates.
URI: https://biore.bio.bg.ac.rs/handle/123456789/5742
ISSN: 0302-8933
DOI: 10.1007/s00203-023-03460-9
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