Please use this identifier to cite or link to this item: https://biore.bio.bg.ac.rs/handle/123456789/4919
Title: The chitinolytic activity of the Curtobacterium sp. isolated from field-grown soybean and analysis of its genome sequence
Authors: Dimkić, Ivica 
Bhardwaj, Vibha
Carpentieri-Pipolo, Valeria
Kuzmanović, Nemanja
Degrassi, Giuliano
Keywords: Chitin;Genomics;Polysaccharides;Soybeans;Carbohydrates;Hydrolases;Phosphorylases;Phylogenetics
Issue Date: 3-Nov-2021
Rank: M22
Publisher: PLOS
Citation: Dimkić I, Bhardwaj V, Carpentieri-Pipolo V, Kuzmanović N, Degrassi G (2021) The chitinolytic activity of the Curtobacterium sp. isolated from field-grown soybean and analysis of its genome sequence. PLoS ONE 16(11): e0259465. https://doi.org/10.1371/journal.pone.0259465
Journal: PLoS One / Public Library of Science
Abstract: 
Curtobacterium sp. GD1 was isolated from leaves of conventionally grown soybean in Brazil. It was noteworthy that among all bacteria previously isolated from the same origin, only Curtobacterium sp. GD1 showed a strong chitinase activity. The enzyme was secreted and its production was induced by the presence of colloidal chitin in the medium. The chitinase was partially purified and characterized: molecular weight was approximately 37 kDa and specific activity 90.8 U/mg. Furthermore, Curtobacterium sp. GD1 genome was sequenced and analyzed. Our isolate formed a phylogenetic cluster with four other Curtobacterium spp. strains, with ANIb/ANIm ≥ 98%, representing a new, still non described Curtobacterium species. The circular genome visualization and comparison of genome sequences of strains forming new cluster indicated that most regions within their genomes were highly conserved. The gene associated with chitinase production was identified and the distribution pattern of glycosyl hydrolases genes was assessed. Also, genes associated with catabolism of structural carbohydrates such as oligosaccharides, mixed polysaccharides, plant and animal polysaccharides, as well as genes or gene clusters associated with resistance to antibiotics, toxic compounds and auxin biosynthesis subsystem products were identified. The abundance of putative glycosyl hydrolases in the genome of Curtobacterium sp. GD1 suggests that it has the tools for the hydrolysis of different polysaccharides. Therefore, Curtobacterium sp. GD1 isolated from soybean might be a bioremediator, biocontrol agent, an elicitor of the plant defense responses or simply degrader.
URI: https://biore.bio.bg.ac.rs/handle/123456789/4919
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0259465
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