Please use this identifier to cite or link to this item: https://biore.bio.bg.ac.rs/handle/123456789/4919
DC FieldValueLanguage
dc.contributor.authorDimkić, Ivicaen_US
dc.contributor.authorBhardwaj, Vibhaen_US
dc.contributor.authorCarpentieri-Pipolo, Valeriaen_US
dc.contributor.authorKuzmanović, Nemanjaen_US
dc.contributor.authorDegrassi, Giulianoen_US
dc.date.accessioned2022-11-04T20:15:19Z-
dc.date.available2022-11-04T20:15:19Z-
dc.date.issued2021-11-03-
dc.identifier.citationDimkić I, Bhardwaj V, Carpentieri-Pipolo V, Kuzmanović N, Degrassi G (2021) The chitinolytic activity of the Curtobacterium sp. isolated from field-grown soybean and analysis of its genome sequence. PLoS ONE 16(11): e0259465. https://doi.org/10.1371/journal.pone.0259465en_US
dc.identifier.issn1932-6203-
dc.identifier.urihttps://biore.bio.bg.ac.rs/handle/123456789/4919-
dc.description.abstractCurtobacterium sp. GD1 was isolated from leaves of conventionally grown soybean in Brazil. It was noteworthy that among all bacteria previously isolated from the same origin, only Curtobacterium sp. GD1 showed a strong chitinase activity. The enzyme was secreted and its production was induced by the presence of colloidal chitin in the medium. The chitinase was partially purified and characterized: molecular weight was approximately 37 kDa and specific activity 90.8 U/mg. Furthermore, Curtobacterium sp. GD1 genome was sequenced and analyzed. Our isolate formed a phylogenetic cluster with four other Curtobacterium spp. strains, with ANIb/ANIm ≥ 98%, representing a new, still non described Curtobacterium species. The circular genome visualization and comparison of genome sequences of strains forming new cluster indicated that most regions within their genomes were highly conserved. The gene associated with chitinase production was identified and the distribution pattern of glycosyl hydrolases genes was assessed. Also, genes associated with catabolism of structural carbohydrates such as oligosaccharides, mixed polysaccharides, plant and animal polysaccharides, as well as genes or gene clusters associated with resistance to antibiotics, toxic compounds and auxin biosynthesis subsystem products were identified. The abundance of putative glycosyl hydrolases in the genome of Curtobacterium sp. GD1 suggests that it has the tools for the hydrolysis of different polysaccharides. Therefore, Curtobacterium sp. GD1 isolated from soybean might be a bioremediator, biocontrol agent, an elicitor of the plant defense responses or simply degrader.en_US
dc.language.isoenen_US
dc.publisherPLOSen_US
dc.relation.ispartofPLoS One / Public Library of Scienceen_US
dc.subjectChitinen_US
dc.subjectGenomicsen_US
dc.subjectPolysaccharidesen_US
dc.subjectSoybeansen_US
dc.subjectCarbohydratesen_US
dc.subjectHydrolasesen_US
dc.subjectPhosphorylasesen_US
dc.subjectPhylogeneticsen_US
dc.titleThe chitinolytic activity of the Curtobacterium sp. isolated from field-grown soybean and analysis of its genome sequenceen_US
dc.typeArticleen_US
dc.identifier.doi10.1371/journal.pone.0259465-
dc.identifier.urlhttps://journals.plos.org/plosone/article?id=10.1371/journal.pone.0259465-
dc.description.rankM22en_US
dc.description.impact3.752en_US
item.languageiso639-1en-
item.cerifentitytypePublications-
item.openairetypeArticle-
item.fulltextNo Fulltext-
item.grantfulltextnone-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
crisitem.author.deptChair of Biochemistry and Molecular Biology-
crisitem.author.orcid0000-0002-0425-5938-
Appears in Collections:Journal Article
Show simple item record

SCOPUSTM   
Citations

12
checked on Nov 20, 2024

Google ScholarTM

Check

Altmetric

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.