Please use this identifier to cite or link to this item: https://biore.bio.bg.ac.rs/handle/123456789/4705
Title: Fluoride subacute testicular toxicity in Wistar rats: Benchmark dose analysis for the redox parameters, essential elements and DNA damage
Authors: Radovanović, Jelena
Antonijević, Biljana
Ćurčić, Marijana
Baralić, Katarina
Kolarević, Stoimir 
Bulat, Zorica
Đukić-Ćosić, Danijela
Buha Djordjević, Aleksandra
Vuković-Gačić, Branka 
Javorac, Dragana
Antonijević Miljaković, Evica
Carević, Momir
Mandinić, Zoran
Keywords: Benchmark methodology;Bioelements;Comet assay;Fluoride;Oxidative stress;Testicles
Issue Date: 1-Dec-2022
Rank: M21
Publisher: Elsevier Ltd
Journal: Environmental Pollution
Abstract: 
Excessive fluoride (F-) levels in the environment could induce different pathological changes, including comorbidities in reproductive functions. Hence, the aim of the present in vivo study was to explore F- subacute toxicity mechanisms via Benchmark dose (BMD) methodology on rat's testicles. The experiment was conducted on thirty male Wistar rats for 28 days, divided into six groups (n = 5): 1) Control (tap water); 2) 10 mg/L F-; 3) 25 mg/L F-; 4) 50 mg/L F-; 5) 100 mg/L F-; 6) 150 mg/L F-. Testicles were dissected out and processed for the determination of F- tissue concentrations, redox status parameters, essential elements level, and DNA damage. PROASTweb 70.1 software was used for determination of external and internal dose-response relationship. The results confirmed a significant increase in superoxide anion (O2.-), total oxidative status (TOS), copper (Cu), zinc (Zn), iron (Fe), DNA damage levels, and decrease in superoxide dismutase activity (SOD1) and total thiol (SH) groups. The dose-dependent changes were confirmed for SOD1 activity and DNA damage. The most sensitive parameters were SOD1 activity and DNA damage with the lowest BMDLs 0.1 μg F-/kg b. w. Since human and animal populations are daily and frequently unconsciously exposed to F-, this dose-response study is valuable for further research regarding the F- health risk assessment.
URI: https://biore.bio.bg.ac.rs/handle/123456789/4705
DOI: 10.1016/j.envpol.2022.120321.
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