Please use this identifier to cite or link to this item: https://biore.bio.bg.ac.rs/handle/123456789/4620
Title: The Influence of Seasonality on Secondary Metabolite Profiles and Neuroprotective Activities of Moss Hypnum cupressiforme Extracts: In Vitro and In Silico Study
Authors: Lunić, Tanja 
Mandić, Marija 
Pavlović Oalđe, Maria 
Sabovljević, Aneta 
Sabovljević, Marko 
Božić-Nedeljković, Biljana 
Božić, Bojan 
Keywords: Bryophytes;Moss extract;Hypnum cupressiforme;Seasonal changes;Secondary metabolites;Anti-inflammatory;Anti-neurodegenerative;Neuroprotection;Molecular docking
Issue Date: 1-Jan-2022
Rank: M21
Publisher: MDPI
Journal: Plants
Abstract: 
Numerous representatives of mosses, including Hypnum cupressiforme, have been used toalleviate different inflammation-related conditions. However, the mode of action underlying thisanti-inflammatory potential has been poorly understood. Moreover, the influence of seasonalityon the chemical composition and biological activity of mosses is generally overlooked. This studyaimed to investigate the influence of seasonal changes (spring, summer, and autumn) on secondarymetabolite composition and biological activities of ethyl acetate H. cupressiforme extracts. Antioxidantactivity was measured usingβ-carotene bleaching assay, while MTT, NBT, ELISA, and Griess assayswere carried out to explore the anti-neuroinflammatory and neuroprotective potential of extracts.Inhibitory activities on acetylcholinesterase and tyrosinase were assessed experimentally and bydocking analysis. The highest content of secondary metabolites and antioxidant activity wereobserved in moss during the summer. Extracts inhibited the secretion of ROS, NO, TNF-α, andIL-6, alleviating the inflammatory potential of H2O2and LPS in microglial and neuronal cells.Strong inhibitory effects on acetylcholinesterase and tyrosinase were observedin vitro. Dockinganalyses revealed high-affinity interactions of secondary metabolites present in H. cupressiforme withimportant enzyme residues. Altogether, these results reveal the neuroprotective potential and thesignificance of seasonal fluctuations on secondary metabolite content and biological activities in mossH. cupressiforme
URI: https://biore.bio.bg.ac.rs/handle/123456789/4620
DOI: 10.3390/plants11010123
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