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Title: | l-Arginine Induces White Adipose Tissue Browning—A New Pharmaceutical Alternative to Cold | Authors: | Kalezic, Andjelika Korać, Aleksandra Korać, Bato Jankovic, Aleksandra. |
Keywords: | Nitric oxide;L-arginine;Browning | Issue Date: | 28-Jun-2022 | Rank: | M21a | Publisher: | MDPI | Journal: | Pharmaceutics | Volume: | 14 | Issue: | 7 | Start page: | 1368 | Abstract: | The beneficial effects of l-arginine supplementation in obesity and type II diabetes involve white adipose tissue (WAT) reduction and increased substrate oxidation. We aimed to test the potential of l-arginine to induce WAT browning. Therefore, the molecular basis of browning was investigated in retroperitoneal WAT (rpWAT) of rats exposed to cold or treated with 2.25% l-arginine for 1, 3, and 7 days. Compared to untreated control, levels of inducible nitric oxide (NO) synthase protein expression and NO signaling increased in both cold-exposed and l-arginine-treated groups. These increases coincided with the appearance of multilocular adipocytes and increased expression levels of uncoupling protein 1 (UCP1), thermogenic and beige adipocyte-specific genes (Cidea, Cd137, and Tmem26), mitochondriogenesis markers (peroxisome proliferator-activated receptor (PPAR)-γ coactivator-1α, mitochondrial DNA copy number), nuclear respiratory factor 1, PPARα and their respective downstream lipid oxidation enzymes after l-arginine treatment. Such browning phenotype in the l-arginine-treated group was concordant with end-course decreases in leptinaemia, rpWAT mass, and body weight. In conclusion, l-arginine mimics cold-mediated increases in NO signaling in rpWAT and induces molecular and structural fingerprints of rpWAT browning. The results endorse l-arginine as a pharmaceutical alternative to cold exposure, which could be of great interest in obesity and associated metabolic diseases. |
URI: | https://biore.bio.bg.ac.rs/handle/123456789/4587 | ISSN: | 1999-4923 | DOI: | 10.3390/pharmaceutics14071368 |
Appears in Collections: | Journal Article |
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