Please use this identifier to cite or link to this item: https://biore.bio.bg.ac.rs/handle/123456789/4583
Title: Effects of Remote Ischaemic Preconditioning on the Internal Thoracic Artery Nitric Oxide Synthase Isoforms in Patients Undergoing Coronary Artery Bypass Grafting
Authors: Janković, Aleksandra
Zakić, Tamara
Miličić, Miroslav
Unić-Stojanović, Dragana
Kalezić, Andjelika
Korać, Aleksandra 
Jović, Miomir
Korać, Bato 
Keywords: Coronary artery graft;Internal thoracic artery;Nitric oxide synthase isoforms;Remote ischaemic preconditioning
Issue Date: 29-Nov-2021
Rank: M21a
Publisher: MDPI
Citation: Jankovic, Aleksandra, Tamara Zakic, Miroslav Milicic, Dragana Unic-Stojanovic, Andjelika Kalezic, Aleksandra Korac, Miomir Jovic, and Bato Korac. 2021. "Effects of Remote Ischaemic Preconditioning on the Internal Thoracic Artery Nitric Oxide Synthase Isoforms in Patients Undergoing Coronary Artery Bypass Grafting" Antioxidants 10, no. 12: 1910. https://doi.org/10.3390/antiox10121910
Journal: Antioxidants
Volume: 10
Issue: 12
Start page: 1910
Abstract: 
Remote ischaemic preconditioning (RIPC) is a medical procedure that consists of repeated brief periods of transient ischaemia and reperfusion of distant organs (limbs) with the ability to provide internal organ protection from ischaemia. Even though RIPC has been successfully applied in patients with myocardial infarction during coronary revascularization (surgery/percutaneous angioplasty), the underlying molecular mechanisms are yet to be clarified. Thus, our study aimed to determine the role of nitric oxide synthase (NOS) isoforms in RIPC-induced protection (3 × 5 min of forearm ischaemia with 5 min of reperfusion) of arterial graft in patients undergoing urgent coronary artery bypass grafting (CABG). We examined RIPC effects on specific expression and immunolocalization of three NOS isoforms - endothelial (eNOS), inducible (iNOS) and neuronal (nNOS) in patients' internal thoracic artery (ITA) used as a graft. We found that the application of RIPC protocol leads to an increased protein expression of eNOS, which was further confirmed with strong eNOS immunopositivity, especially in the endothelium and smooth muscle cells of ITA. The same analysis of two other NOS isoforms, iNOS and nNOS, showed no significant differences between patients undergoing CABG with or without RIPC. Our results demonstrate RIPC-induced upregulation of eNOS in human ITA, pointing to its significance in achieving protective phenotype on a systemic level with important implications for graft patency.
URI: https://biore.bio.bg.ac.rs/handle/123456789/4583
ISSN: 2076-3921
DOI: 10.3390/antiox10121910
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