Please use this identifier to cite or link to this item: https://biore.bio.bg.ac.rs/handle/123456789/3244
Title: Additive and synergistic effects of Bacillus spp. isolates and essential oils on the control of phytopathogenic and saprophytic fungi from medicinal plants and marigold seeds
Authors: Dimkić, Ivica 
Berić, Tanja 
Stević, Tatjana
Pavlović, Snežana
Šavikin, Katarina
Fira, Đorđe 
Stanković, Slaviša 
Keywords: Antifungal activity;Biocontrol;Lipopeptides;Marigold seed;Savory oil;Seed germination;Thyme oil
Issue Date: 1-Aug-2015
Rank: M21
Publisher: Elsevier
Project: Molecular characterization of bacteria from genera Bacillus and Pseudomonas as potential agents for biological control 
Traditional and new products of cultivated and wild growing fruits and grape vines, and by-products durring processing, with special emphasis on indigenous varieties: chemical characterization and biological profile 
Journal: Biological Control
Volume: 87
Start page: 6
End page: 13
Abstract: 
Contamination of plants and seeds with microorganisms is one of the main problems in the production and distribution of various agricultural products, as well as raw herbal material for the preparation of herbal remedies. In targeting microbial contamination, among other bacteria, Bacillus species showed a significant capacity for biocontrol. The antifungal activity of 14 isolates of Bacillus spp. against 15 fungal isolates from medicinal plants was examined utilizing a dual plate assay. The strongest and broadest antagonistic activity against all fungi tested was exhibited by isolates SS-12.6 and SS-13.1 (from a 43% to 74% reduction in fungal growth), while isolates SS-39.1 and SS-39.3 were effective against the fewest fungus species and also had the weakest antifungal activity. The effect of a crude lipopeptide extract (CLE) of Bacillus sp. SS-12.6 was similar to that achieved by a dual culture with isolate SS-12.6, confirming that the antagonism was the result of the antifungal activities of lipopeptides. In addition, essential oils of thyme (0.55. mg/mL) and savory (0.32. mg/mL) in various combinations with the CLE of SS-12.6 were tested for antifungal activity, and additive and synergistic effects for some of the fungi were obtained. When testing the effect of CLE, oils (0.40. mg/mL for thyme oil and 0.21. mg/mL for savory oil) and combinations in situ on marigold seeds, a reduction of total fungal infection without an adverse effect on germination was accomplished by 6-h treatments with CLE of SS-12.6 (85% reduction of fungal infection and 63% germination), supernatant from liquid culture of SS-12.6 (more than 90% reduction of fungal infection with 69% seed germination) and combinations of CLE and savory oil (77% reduction of fungal infection and 62% seed germination) and CLE with thyme and savory oils (about 75% reduction of fungal infection with 69% seed germination).
URI: https://biore.bio.bg.ac.rs/handle/123456789/3244
ISSN: 1049-9644
DOI: 10.1016/j.biocontrol.2015.04.011
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