Please use this identifier to cite or link to this item: https://biore.bio.bg.ac.rs/handle/123456789/2950
Title: STED nanoscopy of the centrosome linker reveals a CEP68-organized, periodic rootletin network anchored to a C-Nap1 ring at centrioles
Authors: Vlijm, Rifka
Li, Xue
Panić, Marko 
Rüthnick, Diana
Hata, Shoji
Herrmannsdörfer, Frank
Kuner, Thomas
Heilemann, Mike
Engelhardt, Johann
Hell, Stefan W.
Schiebel, Elmar
Keywords: Centrosome;Centrosome linker;CEP68;Rootletin;STED superresolution
Issue Date: 6-Mar-2018
Rank: M21a
Journal: Proceedings of the National Academy of Sciences of the United States of America
Abstract: 
© 2018 National Academy of Sciences. All Rights Reserved. The centrosome linker proteins C-Nap1, rootletin, and CEP68 connect the two centrosomes of a cell during interphase into one microtubule-organizing center. This coupling is important for cell migration, cilia formation, and timing of mitotic spindle formation. Very little is known about the structure of the centrosome linker. Here, we used stimulated emission depletion (STED) microscopy to show that each C-Nap1 ring at the proximal end of the two centrioles organizes a rootletin ring and, in addition, multiple rootletin/CEP68 fibers. Rootletin/CEP68 fibers originating from the two centrosomes form a web-like, interdigitating network, explaining the flexible nature of the centrosome linker. The rootletin/CEP68 filaments are repetitive and highly ordered. Staggered rootletin molecules (N-to-N and C-to-C) within the filaments are 75 nm apart. Rootletin binds CEP68 via its C-terminal spectrin repeat-containing region in 75-nm intervals. The N-to-C distance of two rootletin molecules is ?35 to 40 nm, leading to an estimated minimal rootletin length of ?110 nm. CEP68 is important in forming rootletin filaments that branch off centrioles and to modulate the thickness of rootletin fibers. Thus, the centrosome linker consists of a vast network of repeating rootletin units with C-Nap1 as ring organizer and CEP68 as filament modulator.
URI: https://biore.bio.bg.ac.rs/handle/123456789/2950
ISSN: 0027-8424
DOI: 10.1073/pnas.1716840115
Appears in Collections:Journal Article

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