Please use this identifier to cite or link to this item: https://biore.bio.bg.ac.rs/handle/123456789/2950
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dc.contributor.authorVlijm, Rifkaen_US
dc.contributor.authorLi, Xueen_US
dc.contributor.authorPanić, Markoen_US
dc.contributor.authorRüthnick, Dianaen_US
dc.contributor.authorHata, Shojien_US
dc.contributor.authorHerrmannsdörfer, Franken_US
dc.contributor.authorKuner, Thomasen_US
dc.contributor.authorHeilemann, Mikeen_US
dc.contributor.authorEngelhardt, Johannen_US
dc.contributor.authorHell, Stefan W.en_US
dc.contributor.authorSchiebel, Elmaren_US
dc.date.accessioned2019-10-29T17:37:34Z-
dc.date.available2019-10-29T17:37:34Z-
dc.date.issued2018-03-06-
dc.identifier.issn0027-8424-
dc.identifier.urihttps://biore.bio.bg.ac.rs/handle/123456789/2950-
dc.description.abstract© 2018 National Academy of Sciences. All Rights Reserved. The centrosome linker proteins C-Nap1, rootletin, and CEP68 connect the two centrosomes of a cell during interphase into one microtubule-organizing center. This coupling is important for cell migration, cilia formation, and timing of mitotic spindle formation. Very little is known about the structure of the centrosome linker. Here, we used stimulated emission depletion (STED) microscopy to show that each C-Nap1 ring at the proximal end of the two centrioles organizes a rootletin ring and, in addition, multiple rootletin/CEP68 fibers. Rootletin/CEP68 fibers originating from the two centrosomes form a web-like, interdigitating network, explaining the flexible nature of the centrosome linker. The rootletin/CEP68 filaments are repetitive and highly ordered. Staggered rootletin molecules (N-to-N and C-to-C) within the filaments are 75 nm apart. Rootletin binds CEP68 via its C-terminal spectrin repeat-containing region in 75-nm intervals. The N-to-C distance of two rootletin molecules is ?35 to 40 nm, leading to an estimated minimal rootletin length of ?110 nm. CEP68 is important in forming rootletin filaments that branch off centrioles and to modulate the thickness of rootletin fibers. Thus, the centrosome linker consists of a vast network of repeating rootletin units with C-Nap1 as ring organizer and CEP68 as filament modulator.en_US
dc.language.isoenen_US
dc.relation.ispartofProceedings of the National Academy of Sciences of the United States of Americaen_US
dc.subjectCentrosomeen_US
dc.subjectCentrosome linkeren_US
dc.subjectCEP68en_US
dc.subjectRootletinen_US
dc.subjectSTED superresolutionen_US
dc.titleSTED nanoscopy of the centrosome linker reveals a CEP68-organized, periodic rootletin network anchored to a C-Nap1 ring at centriolesen_US
dc.typeArticleen_US
dc.identifier.doi10.1073/pnas.1716840115-
dc.identifier.pmid29463719-
dc.identifier.scopus2-s2.0-85042942912-
dc.identifier.urlhttps://api.elsevier.com/content/abstract/scopus_id/85042942912-
dc.description.rankM21a-
dc.description.impact12.291-
item.languageiso639-1en-
item.cerifentitytypePublications-
item.openairetypeArticle-
item.fulltextWith Fulltext-
item.grantfulltextrestricted-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
crisitem.author.deptChair of Biochemistry and Molecular Biology-
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