Please use this identifier to cite or link to this item:
https://biore.bio.bg.ac.rs/handle/123456789/2949
Title: | Human phosphatase CDC14A regulates actin organization through dephosphorylation of epithelial protein lost in neoplasm | Authors: | Chen, Nan Peng Uddin, Borhan Hardt, Robert Ding, Wen Panić, Marko Lucibello, Ilaria Kammerer, Patricia Ruppert, Thomas Schiebel, Elmar |
Keywords: | Actin dynamics;CDC14;Eplin | Issue Date: | 16-May-2017 | Journal: | Proceedings of the National Academy of Sciences of the United States of America | Abstract: | CDC14 is an essential dual-specificity phosphatase that counteracts CDK1 activity during anaphase to promote mitotic exit in Saccharomyces cerevisiae. Surprisingly, human CDC14A is not essential for cell cycle progression. Instead, it regulates cell migration and cell adhesion. Little is known about the substrates of hCDC14A and the counteracting kinases. Here, we combine phospho-proteome profiling and proximity-dependent biotin identification to identify hCDC14A substrates. Among these targets were actin regulators, including the tumor suppressor eplin. hCDC14A counteracts EGFinduced rearrangements of actin cytoskeleton by dephosphorylating eplin at two known extracellular signal-regulated kinase sites, serine 362 and 604. hCDC14APD and eplin knockout cell lines exhibited down-regulation of E-cadherin and a reduction in α/β-catenin at cell-cell adhesions. Reduction in the levels of hCDC14A and eplin mRNA is frequently associated with colorectal carcinoma and is correlated with poor prognosis. We therefore propose that eplin dephosphorylation by hCDC14A reduces actin dynamics to restrict tumor malignancy. |
URI: | https://biore.bio.bg.ac.rs/handle/123456789/2949 | ISSN: | 0027-8424 | DOI: | 10.1073/pnas.1619356114 |
Appears in Collections: | Journal Article |
Files in This Item:
File | Description | Size | Format | Existing users please |
---|---|---|---|---|
Chen et al. 2017 PNAS.pdf | 1.63 MB | Adobe PDF | Request a copy |
SCOPUSTM
Citations
24
checked on Dec 22, 2024
Page view(s)
2
checked on Dec 23, 2024
Google ScholarTM
Check
Altmetric
Altmetric
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.