Please use this identifier to cite or link to this item: https://biore.bio.bg.ac.rs/handle/123456789/2263
Title: Identification of seed coat phenolic compounds from differently colored pea varieties and characterization of their antioxidant activity
Authors: Stanisavljević, Nemanja S.
Ilić, Marija
Jovanović, Živko 
Čupić, Tihomir
Dabić, Dragana C.
Natić, Maja M.
Tešić, Živoslav Lj
Radović, Svetlana 
Keywords: Antioxidant;Phenolic content;Pisum sativum L.;Seed coat;UHPLC-LTQ OrbiTrap MS
Issue Date: 1-Jan-2015
Journal: Archives of Biological Sciences
Abstract: 
The phenolic composition of seed coats in four differently colored pea varieties (Pisum sativum L.) was investigated using UHPLC-LTQ OrbiTrap MS. The obtained findings revealed that the seed coats of the examined pea genotypes possess a unique phenolic composition compared to previously studied European cultivars. In total, 41 phenolic compounds have been identified. The seed coats of the studied cultivars contained certain amounts of rosmarinic acid, rutin, galangin, morin, naringin, hesperetin and pinocembrin as well as ten flavonol glycosides that had not been reported previously. Additionally, the total phenolic content, antioxidant activity and metal chelating capacity of extracts was determined using Folin-Ciocalteu's method, 2,2-diphenyl-1-picrylhydrazyl radical scavenging assay, ferric ion-reducing capacity and ferrous ion-chelating capacity assay, respectively. Dark colored genotypes MBK 168 and MBK 173 possessed the highest total phenolic contents as well the strongest antioxidant activities. On the other hand, bright colored genotypes MBK 88 and MBK 90 exhibited the strongest metal-chelating capacities. The examined pea seed coats may be considered as important potential contributors to human health due to the presence of bioactive phenolic constituents. In addition, our results could be used as a guideline for breeding new pea cultivars with high antioxidant activities applicable in the formulation of functional food products.
URI: https://biore.bio.bg.ac.rs/handle/123456789/2263
ISSN: 0354-4664
DOI: 10.2298/ABS141204042S
Appears in Collections:Journal Article

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