Please use this identifier to cite or link to this item: https://biore.bio.bg.ac.rs/handle/123456789/1540
Title: Polarography as a technique of choice for the evaluation of total antioxidant activity: The case study of selected Coprinus Comatus extracts and quinic acid, their antidiabetic ingredient
Authors: Karaman, Maja
Tesanovic, Kristina 
Gorjanovic, Stanislava
Pastor, Ferenc T.
Simonovic, Mladen
Glumac, Miodrag
Pejin, Boris
Keywords: antioxidativity;cyclic polyols;HPMC assay;polarography;quinic acid
Issue Date: 1-Jan-2019
Rank: M22
Journal: Natural Product Research
Abstract: 
© 2019, © 2019 Informa UK Limited, trading as Taylor & Francis Group. This study was focused on in vitro screening of the total antioxidant activity of the selected extracts of the mushroom Coprinus comatus and quinic acid, one of their antidiabetic ingredients, by an uncommon electrochemical assay. Indeed, direct current (DC) polarographic HydroxoPerhydroxo Mercury(II) Complex (HPMC) assay based on decrease of anodic limiting current originating from HPMC formation in alkaline solutions of hydrogen peroxide at potential of mercury dissolution, observed upon gradual addition of antioxidants, was applied herein for the estimation of the natural products' antioxidativity. Quinic acid was found to exhibit most promising antioxidant potential (4.0 ± 0.2%µL−1) being ≈ 2-fold more active than the screened C. comatus extract samples. Actually, such a finding puts some light on the antioxidativity of cyclic polyols, well understimated class of organic compounds, compared to aromatic (poly)phenolics. As a low cost, easy-to handle and accurate this polarographic assay may be thoroughly recommended for much broader use.
URI: https://biore.bio.bg.ac.rs/handle/123456789/1540
ISSN: 1478-6419
DOI: 10.1080/14786419.2019.1628753
Appears in Collections:Journal Article

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