Please use this identifier to cite or link to this item: https://biore.bio.bg.ac.rs/handle/123456789/1432
Title: Comparative study of the antimutagenic potential of Vitamin E in different E. coli strains
Authors: Nikolić, Biljana 
Stanojević, Jasna
Mitić Ćulafić, Dragana 
Vuković Gačić, Branka 
Knežević Vukčević, Jelena 
Simić, Draga
Keywords: Antimutagenesis;WP2 antimutagenicity test;Vitamin E;E. coli K12 assay-system;Antioxidant
Issue Date: 14-Nov-2004
Journal: Mutation Research - Genetic Toxicology and Environmental Mutagenesis
Abstract: 
The antimutagenic potential of Vitamin E due to its antioxidative properties was studied. The new Escherichia coli K12 assay-system designed in our laboratory was employed in order to detect the antimutagenic potential of Vitamin E and to determine its molecular mechanisms of action. The assay is composed of three tests. In Test A, we examine the influence of the antioxidant on induced oxidative mutagenesis in a repair-proficient strain. Spontaneous mutagenesis is monitored in Test B, which is performed with two mutator strains, one mismatch repair-deficient (mutS) and another deficient in 8-oxo-dGTP-ase activity (mutT). In Test M, a repair-proficient strain and its mismatch repair-deficient counterpart (mutH), both carrying a plasmid with microsatellite sequences, are used to measure the level of microsatellite instability. To examine the antimutagenic potential of Vitamin E we also used the WP2 antimutagenicity test. Protective properties of Vitamin E against oxidative mutagenesis were detected in all tests with the E. coli K12 assay-system as well as in the WP2 antimutagenicity test. This study confirms that mismatch repair is essential for repair of oxidative DNA damage. The results obtained indicate that Vitamin E prevents the formation of DNA adducts by lipid peroxidation products rather than those formed by direct oxidation of DNA bases. Moreover, it can reduce microsatellite instability. After further validation, the new E. coli K12 assay-system can be used to test the antimutagenic potential of antioxidants. © 2004 Elsevier B.V. All rights reserved.
URI: https://biore.bio.bg.ac.rs/handle/123456789/1432
ISSN: 1383-5718
DOI: 10.1016/j.mrgentox.2004.07.007
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