Please use this identifier to cite or link to this item: https://biore.bio.bg.ac.rs/handle/123456789/1432
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dc.contributor.authorNikolić, Biljanaen_US
dc.contributor.authorStanojević, Jasnaen_US
dc.contributor.authorMitić Ćulafić, Draganaen_US
dc.contributor.authorVuković Gačić, Brankaen_US
dc.contributor.authorKnežević Vukčević, Jelenaen_US
dc.contributor.authorSimić, Dragaen_US
dc.date.accessioned2019-09-26T10:07:28Z-
dc.date.available2019-09-26T10:07:28Z-
dc.date.issued2004-11-14-
dc.identifier.issn1383-5718-
dc.identifier.urihttps://biore.bio.bg.ac.rs/handle/123456789/1432-
dc.description.abstractThe antimutagenic potential of Vitamin E due to its antioxidative properties was studied. The new Escherichia coli K12 assay-system designed in our laboratory was employed in order to detect the antimutagenic potential of Vitamin E and to determine its molecular mechanisms of action. The assay is composed of three tests. In Test A, we examine the influence of the antioxidant on induced oxidative mutagenesis in a repair-proficient strain. Spontaneous mutagenesis is monitored in Test B, which is performed with two mutator strains, one mismatch repair-deficient (mutS) and another deficient in 8-oxo-dGTP-ase activity (mutT). In Test M, a repair-proficient strain and its mismatch repair-deficient counterpart (mutH), both carrying a plasmid with microsatellite sequences, are used to measure the level of microsatellite instability. To examine the antimutagenic potential of Vitamin E we also used the WP2 antimutagenicity test. Protective properties of Vitamin E against oxidative mutagenesis were detected in all tests with the E. coli K12 assay-system as well as in the WP2 antimutagenicity test. This study confirms that mismatch repair is essential for repair of oxidative DNA damage. The results obtained indicate that Vitamin E prevents the formation of DNA adducts by lipid peroxidation products rather than those formed by direct oxidation of DNA bases. Moreover, it can reduce microsatellite instability. After further validation, the new E. coli K12 assay-system can be used to test the antimutagenic potential of antioxidants. © 2004 Elsevier B.V. All rights reserved.en_US
dc.language.isoenen_US
dc.relation.ispartofMutation Research - Genetic Toxicology and Environmental Mutagenesisen_US
dc.subjectAntimutagenesisen_US
dc.subjectWP2 antimutagenicity testen_US
dc.subjectVitamin Een_US
dc.subjectE. coli K12 assay-systemen_US
dc.subjectAntioxidanten_US
dc.titleComparative study of the antimutagenic potential of Vitamin E in different E. coli strainsen_US
dc.typeArticleen_US
dc.identifier.doi10.1016/j.mrgentox.2004.07.007-
dc.identifier.pmid15474408-
dc.identifier.scopus2-s2.0-5444244449-
dc.identifier.urlhttps://api.elsevier.com/content/abstract/scopus_id/5444244449-
item.languageiso639-1en-
item.cerifentitytypePublications-
item.openairetypeArticle-
item.fulltextNo Fulltext-
item.grantfulltextnone-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
crisitem.author.deptChair of Microbiology-
crisitem.author.deptChair of Microbiology-
crisitem.author.deptChair of Microbiology-
crisitem.author.deptChair of Microbiology-
crisitem.author.orcid0000-0003-1765-2454-
crisitem.author.orcid0000-0002-6651-6814-
crisitem.author.orcid0000-0001-8767-1912-
crisitem.author.orcid0000-0002-8138-6579-
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