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Title: | Protective effect of basil (Ocimum basilicum L.) against oxidative DNA damage and mutagenesis | Authors: | Berić, Tanja Nikolić, Biljana Stanojević, Jasna Vuković Gačić, Branka Knežević Vukčević, Jelena |
Keywords: | Basil;Yeast comet assay;Reversion tests;Oxidative DNA damage | Issue Date: | 1-Feb-2008 | Journal: | Food and Chemical Toxicology | Abstract: | Mutagenic and antimutagenic properties of essential oil (EO) of basil and its major constituent Linalool, reported to possess antioxidative properties, were examined in microbial tests. In Salmonella/microsome and Escherichia. coli WP2 reversion assays both derivatives (0.25-2.0 μl/plate) showed no mutagenic effect. Salmonella. typhimurium TA98, TA100 and TA102 strains displayed similar sensitivity to both basil derivatives as non-permeable E. coli WP2 strains IC185 and IC202 oxyR. Moreover, the toxicity of basil derivatives to WP2 strains did not depend on OxyR function. The reduction of t-BOOH-induced mutagenesis by EO and Linalool (30-60%) was obtained in repair proficient strains of the E. coli K12 assay (Nikolić, B., Stanojević, J., Mitić, D., Vuković-Gačić, B., Knežević-Vukčević, J., Simić, D., 2004. Comparative study of the antimutagenic potential of vitamin E in different E. coli strains. Mutat. Res. 564, 31-38), as well as in E. coli WP2 IC202 strain. EO and Linalool reduced spontaneous mutagenesis in mismatch repair deficient E. coli K12 strains (27-44%). In all tests, antimutagenic effect of basil derivatives was comparable with that obtained with model antioxidant vitamin E. Linalool and vitamin E induced DNA strand breaks in Comet assay on S. cerevisiae 3A cells, but at non-genotoxic concentrations (0.075 and 0.025 μg/ml, respectively) they reduced the number of H2O2-induced comets (45-70% Linalool and 80-93% vitamin E). Obtained results indicate that antigenotoxic potential of basil derivatives could be attributed to their antioxidative properties. © 2007 Elsevier Ltd. All rights reserved. |
URI: | https://biore.bio.bg.ac.rs/handle/123456789/1431 | ISSN: | 0278-6915 | DOI: | 10.1016/j.fct.2007.09.102 |
Appears in Collections: | Journal Article |
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