Please use this identifier to cite or link to this item:
https://biore.bio.bg.ac.rs/handle/123456789/140
Title: | Molecular basis of hippocampal energy metabolism in diabetic rats: The effects of SOD mimic | Authors: | Stančić, Ana Otašević, Vesna Janković, Aleksandra Vučetić, Milica Ivanović-Burmazović, Ivana Filipović, Miloš R. Korać, Aleksandra Markelić, Milica Veličković, Ksenija Golić, Igor Buzadžić, Biljana Korać, Bato |
Keywords: | Diabetes;Glycolysis;hippocampus;Oxidative phosphorylation;SOD mimic | Issue Date: | 1-Oct-2013 | Rank: | M22 | Project: | White or/and brown: importance of adipose tissue in overall redox dependent metabolic control in physiological adaptations and metabolic disorders | Journal: | Brain Research Bulletin | Abstract: | Hippocampal structural changes associated with diabetes-related cognitive impairments are well described, but their molecular background remained vague. We examined whether/how diabetes alters molecular basis of energy metabolism in hippocampus readily after diabetes onset, with special emphasis on its redox-sensitivity.To induce diabetes, adult Mill Hill hybrid hooded rats received a single alloxan dose (120mg/kg). Both non-diabetic and diabetic groups were further divided in two subgroups receiving (i) or not (ii) superoxide dismutase (SOD) mimic, [Mn(II)(pyane)Cl2] for 7 days, i.p. Treatment of the diabetic animals started after blood glucose level ≥12mM.Diabetes decreased protein levels of oxidative phosphorylation components: complex III and ATP synthase. In contrast, protein amounts of glyceraldehyde-3-phosphate dehydrogenase, pyruvate dehydrogenase, and hypoxia-inducible factor-1α - the key regulator of energy metabolism in stress conditions, were higher in diabetic animals. Treatment with SOD mimic restored/increased the levels of oxidative phosphorylation components and returned hypoxia-inducible factor-1α to control level, while diabetes-induced up-regulation of glycolytic enzyme, glyceraldehyde-3-phosphate dehydrogenase, was additionally stimulated.To conclude, our results provide insight into the earliest molecular changes of energy-producing pathways in diabetes that may account for structural/functional disturbance of hippocampus, seen during disease progression. Also, data suggest [Mn(II)(pyane)Cl2] as potential therapeutic agent in cutting-edge approaches to threat this widespread metabolic disorder. © 2013 Elsevier Inc. |
URI: | https://biore.bio.bg.ac.rs/handle/123456789/140 | ISSN: | 0361-9230 | DOI: | 10.1016/j.brainresbull.2013.09.009 |
Appears in Collections: | Journal Article |
Show full item record
SCOPUSTM
Citations
16
checked on Oct 31, 2024
Page view(s)
1
checked on Nov 4, 2024
Google ScholarTM
Check
Altmetric
Altmetric
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.