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Title: | Single and combined effects of acute and chronic non-thermal stressors on rat interscapular brown adipose tissue metabolic activity | Authors: | Cvijić, Gordana Lakić, Iva Vujović, Predrag Jasnić, Nebojša Đurašević, Siniša Dronjak-Čučaković, Slađana Đorđević, Jelena |
Keywords: | Catalase;IBAT;Immobilization;Isolation;MAO-A;SOD;UCP-1 | Issue Date: | 27-Aug-2013 | Journal: | Archives of Biological Sciences | Abstract: | The aim of this study was to examine whether the thermogenic potential of rat interscapular brown adipose tissue (IBAT) changes in response to acute and/or chronic exposure to non-thermal stressors (immobilization and isolation), by measuring the uncoupling protein 1 (UCP-1) content, MAO-A, SOD and CAT activities, as well as the number of IBAT sympathetic noradrenaline-containing nerve fibers. Both acute immobilization (2 h) and chronic isolation (21 days), as well as their combined effects, significantly increased the IBAT UCP-1 content in comparison to non-stressed animals. When applied individually, stressors increased the number of sympathetic fibers in comparison to controls, whereas in combination they decreased it. The activity of IBAT monoamine oxidase-A (MAO-A) decreased under the influence of each stressor independent of its type or duration. SOD activity coincided with MAO-A decrement, whereas CAT activity had an opposite pattern of changes. We conclude that acute and chronic exposure to non-thermal stressors, immobilization and isolation, respectively, affect the metabolic potential of rat IBAT, judging by the increase in UCP-1 content and sympathetic outflow. However, when acute immobilization was applied as a novel stressor to previously chronically isolated animals, an increase in the UCP-1 content was accompanied by a lower IBAT sympathetic outflow, suggesting that IBAT metabolic function under various stress condition is not solely dependent on SNS activity. |
URI: | https://biore.bio.bg.ac.rs/handle/123456789/121 | ISSN: | 0354-4664 | DOI: | 10.2298/ABS1303919C |
Appears in Collections: | Journal Article |
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