Please use this identifier to cite or link to this item: https://biore.bio.bg.ac.rs/handle/123456789/112
Title: The origin of lipofuscin in brown adipocytes of hyperinsulinaemic rats: The role of lipid peroxidation and iron
Authors: Markelić, Milica 
Veličković, Ksenija 
Golić, Igor 
Klepal, Waltraud
Otasevic, Vesna
Stancic, Ana
Jankovic, Aleksandra
Vucetic, Milica
Buzadzic, Biljana
Korać, Bato 
Korać, Aleksandra 
Keywords: Brown adipocyte;Hyperinsulinaemia;Iron;Lipofuscin
Issue Date: 15-Mar-2013
Rank: M21
Project: White or/and brown: importance of adipose tissue in overall redox dependent metabolic control in physiological adaptations and metabolic disorders 
Journal: Histology and Histopathology
Abstract: 
The aim of this study was to investigate lipofuscin origin in brown adipocytes of hyperinsulinaemic rats and the possible role of lipid peroxidation and iron in this process. Ultrastructural examination revealed hyperinsulinaemia-induced enhancement in the lipofuscin production, accompanied by an increase of mitochondrial damage in brown adipocytes. Extensive fusions of lipid droplets and mitochondria with lysosomes were also observed. Confocal microscopy showed lipofuscin auto-fluorescence emission in brown adipose tissue (BAT) after excitation at 488 nm and 633 nm, particularly in the insulin-treated groups. The presence and distribution of lipid peroxidation product, 4-hydroxy-2-nonenal (4-HNE), in brown adipocytes was assessed by immunohistochemical examination revealing its higher content after treatment with insulin. The iron content was quantified by electron dispersive X-ray analysis (EDX) showing its higher content in the hyperinsulinaemic groups. The ultrastucture of the majority of lipofuscin granules suggests their mitochondrial origin, which was additionally confirmed by their colocalization with ATP synthase. In conclusion, our results suggest that increased lipofuscinogenesis in the brown adipocytes of hyperinsulinaemic rats is a consequence of lipid peroxidation, mitochondrial damage and iron accumulation.
URI: https://biore.bio.bg.ac.rs/handle/123456789/112
ISSN: 0213-3911
DOI: 10.14670/HH-28.493
Appears in Collections:Journal Article

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