Please use this identifier to cite or link to this item: https://biore.bio.bg.ac.rs/handle/123456789/112
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dc.contributor.authorMarkelić, Milicaen_US
dc.contributor.authorVeličković, Ksenijaen_US
dc.contributor.authorGolić, Igoren_US
dc.contributor.authorKlepal, Waltrauden_US
dc.contributor.authorOtasevic, Vesnaen_US
dc.contributor.authorStancic, Anaen_US
dc.contributor.authorJankovic, Aleksandraen_US
dc.contributor.authorVucetic, Milicaen_US
dc.contributor.authorBuzadzic, Biljanaen_US
dc.contributor.authorKorać, Batoen_US
dc.contributor.authorKorać, Aleksandraen_US
dc.date.accessioned2019-06-20T11:08:45Z-
dc.date.available2019-06-20T11:08:45Z-
dc.date.issued2013-03-15-
dc.identifier.issn0213-3911-
dc.identifier.urihttps://biore.bio.bg.ac.rs/handle/123456789/112-
dc.description.abstractThe aim of this study was to investigate lipofuscin origin in brown adipocytes of hyperinsulinaemic rats and the possible role of lipid peroxidation and iron in this process. Ultrastructural examination revealed hyperinsulinaemia-induced enhancement in the lipofuscin production, accompanied by an increase of mitochondrial damage in brown adipocytes. Extensive fusions of lipid droplets and mitochondria with lysosomes were also observed. Confocal microscopy showed lipofuscin auto-fluorescence emission in brown adipose tissue (BAT) after excitation at 488 nm and 633 nm, particularly in the insulin-treated groups. The presence and distribution of lipid peroxidation product, 4-hydroxy-2-nonenal (4-HNE), in brown adipocytes was assessed by immunohistochemical examination revealing its higher content after treatment with insulin. The iron content was quantified by electron dispersive X-ray analysis (EDX) showing its higher content in the hyperinsulinaemic groups. The ultrastucture of the majority of lipofuscin granules suggests their mitochondrial origin, which was additionally confirmed by their colocalization with ATP synthase. In conclusion, our results suggest that increased lipofuscinogenesis in the brown adipocytes of hyperinsulinaemic rats is a consequence of lipid peroxidation, mitochondrial damage and iron accumulation.en_US
dc.language.isoenen_US
dc.relationWhite or/and brown: importance of adipose tissue in overall redox dependent metabolic control in physiological adaptations and metabolic disordersen_US
dc.relation.ispartofHistology and Histopathologyen_US
dc.subjectBrown adipocyteen_US
dc.subjectHyperinsulinaemiaen_US
dc.subjectIronen_US
dc.subjectLipofuscinen_US
dc.titleThe origin of lipofuscin in brown adipocytes of hyperinsulinaemic rats: The role of lipid peroxidation and ironen_US
dc.typeArticleen_US
dc.identifier.doi10.14670/HH-28.493-
dc.identifier.pmid23335278-
dc.identifier.scopus2-s2.0-84876436813-
dc.identifier.urlhttps://api.elsevier.com/content/abstract/scopus_id/84876436813-
dc.description.rankM21en_US
dc.description.impact2.281en_US
item.languageiso639-1en-
item.cerifentitytypePublications-
item.openairetypeArticle-
item.fulltextNo Fulltext-
item.grantfulltextnone-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
crisitem.author.deptChair of Cell and Tissue Biology-
crisitem.author.deptChair of Cell and Tissue Biology-
crisitem.author.deptChair of Cell and Tissue Biology-
crisitem.author.deptChair of Cell and Tissue Biology-
crisitem.author.orcid0000-0002-5444-7735-
crisitem.author.orcid0000-0002-4373-5483-
crisitem.author.orcid0000-0001-5944-5053-
crisitem.author.orcid0000-0001-5272-579X-
crisitem.author.orcid0000-0002-3044-9963-
crisitem.project.funderMESTD-
crisitem.project.grantno173055-
crisitem.project.fundingProgramBasic Research (BR or ON)-
crisitem.project.openAireinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173055-
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