Please use this identifier to cite or link to this item: https://biore.bio.bg.ac.rs/handle/123456789/1054
Title: Single-neuron level one-photon voltage imaging with sparsely targeted genetically encoded voltage indicators
Authors: Quicke, Peter
Song, Chenchen
McKimm, Eric J.
Milošević, Milena 
Howe, Carmel L.
Neil, Mark
Schultz, Simon R.
Antic, Srdjan D.
Foust, Amanda J.
Knöpfel, Thomas
Keywords: Cerebral cortex;Optogenetics;Sparse expression;Transgenic;Voltage imaging
Issue Date: 29-Jan-2019
Rank: M21
Journal: Frontiers in Cellular Neuroscience
Abstract: 
© 2019 Quicke, Song, McKimm, Milosevic, Howe, Neil, Schultz, Antic, Foust and Knöpfel. Voltage imaging of many neurons simultaneously at single-cell resolution is hampered by the difficulty of detecting small voltage signals from overlapping neuronal processes in neural tissue. Recent advances in genetically encoded voltage indicator (GEVI) imaging have shown single-cell resolution optical voltage recordings in intact tissue through imaging naturally sparse cell classes, sparse viral expression, soma restricted expression, advanced optical systems, or a combination of these. Widespread sparse and strong transgenic GEVI expression would enable straightforward optical access to a densely occurring cell type, such as cortical pyramidal cells. Here we demonstrate that a recently described sparse transgenic expression strategy can enable single-cell resolution voltage imaging of cortical pyramidal cells in intact brain tissue without restricting expression to the soma. We also quantify the functional crosstalk in brain tissue and discuss optimal imaging rates to inform future GEVI experimental design.
URI: https://biore.bio.bg.ac.rs/handle/123456789/1054
ISSN: 1662-5102
DOI: 10.3389/fncel.2019.00039
Appears in Collections:Journal Article

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