Please use this identifier to cite or link to this item:
Title: 17β-Estradiol upregulates ecto-5'-nucleotidase (CD73) in hippocampal synaptosomes of female rats through action mediated by estrogen receptor-α and -β
Authors: Mitrović, N.
Zarić, M.
Drakulić, D.
Martinović, J.
Stanojlović, M.
Sévigny, J.
Horvat, A.
Nedeljković, Nadežda 
Grković, I.
Keywords: 17β-estradiol;Ecto-5'-nucleotidase (CD73);Estradiol receptors α and β;Hippocampus;Synaptosomes
Issue Date: 2-Jun-2016
Journal: Neuroscience
© 2016 IBRO. 17β-Estradiol (E2) crucially affects several processes in the hippocampus of both sexes. E2 acts upon estradiol receptors ERα and ERβ, influencing target gene expression and/or modulates intracellular signaling cascades. Another potent modulator of hippocampal function is nucleoside adenosine, the final product of ectonucleotidase cascade, enzymes which hydrolyze extracellular ATP to adenosine. The last and rate-limiting step of the hydrolysis is catalyzed by membrane-bound ecto-5'-nucleotidase (eN). Previous findings obtained on adenosine metabolism in brain suggest that eN may be modulated by ovarian steroids. Therefore, the present study reports that the activity and protein abundance of membrane-bound eN fluctuates across the estrus cycle in the hippocampal synaptosomes of female rats. Further, we analyzed the role of E2 and its intracellular receptors on the expression of eN in ovariectomized females. We found that E2 upregulated eN activity and protein abundance in the hippocampal synaptosomes. Application of nonspecific ER antagonist, ICI 182,780 and selective ERα and ERβ agonists, PPT and DPN, respectively, demonstrated the involvement of both receptor subtypes in observed actions. Selective ERα receptor agonist, PPT, induced upregulation of both the protein level and activity of eN, while application of selective ERβ receptor agonist, DPN, increased only the activity of eN. In both cases, E2 entered into the intracellular compartment and activated ER(s), which was demonstrated by membrane impermeable E2-BSA conjugate. Together these results imply that E2-induced effects on connectivity and functional properties of the hippocampal synapses may be in part mediated through observed effect on eN.
ISSN: 03064522
DOI: 10.1016/j.neuroscience.2016.03.022
Appears in Collections:Journal Article

Files in This Item:
File Description SizeFormat Existing users please Login
14 17b-ESTRADIOL UPREGULATES ECTO-5-NUCLEOTIDASE (CD73) IN.pdf834.47 kBAdobe PDF    Request a copy
Show full item record


checked on Sep 24, 2021

Page view(s)

checked on May 3, 2021

Google ScholarTM




Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.