Please use this identifier to cite or link to this item: https://biore.bio.bg.ac.rs/handle/123456789/1005
Title: Voltage Gated Potassium Channel Kv1.3 Is Upregulated on Activated Astrocytes in Experimental Autoimmune Encephalomyelitis
Authors: Bozic, Iva
Tesovic, Katarina
Laketa, Danijela 
Adžić, Marija 
Jakovljevic, Marija
Bjelobaba, Ivana
Savic, Danijela
Nedeljković, Nadežda 
Pekovic, Sanja
Lavrnja, Irena
Keywords: Agitoxin-2;Astrocytes;Experimental autoimmune encephalomyelitis;Neuroinflammation;Voltage gated potassium channel Kv1.3
Issue Date: 1-May-2018
Rank: M22
Journal: Neurochemical Research
Abstract: 
© 2018, Springer Science+Business Media, LLC, part of Springer Nature. Kv1.3 is a voltage gated potassium channel that has been implicated in pathophysiology of multiple sclerosis (MS). In the present study we investigated temporal and cellular expression pattern of this channel in the lumbar part of spinal cords of animals with experimental autoimmune encephalomyelitis (EAE), animal model of MS. EAE was actively induced in female Dark Agouti rats. Expression of Kv1.3 was analyzed at different time points of disease progression, at the onset, peak and end of EAE. We here show that Kv1.3 increased by several folds at the peak of EAE at both gene and protein level. Double immunofluorescence analyses demonstrated localization of Kv1.3 on activated microglia, macrophages, and reactive astrocytes around inflammatory lesions. In vitro experiments showed that pharmacological block of Kv1.3 in activated astrocytes suppresses the expression of proinflammatory mediators, suggesting a role of this channel in inflammation. Our results support the hypothesis that Kv1.3 may be a therapeutic target of interest for MS and add astrocytes to the list of cells whose activation would be suppressed by inhibiting Kv1.3 in inflammatory conditions.
URI: https://biore.bio.bg.ac.rs/handle/123456789/1005
ISSN: 0364-3190
DOI: 10.1007/s11064-018-2509-8
Appears in Collections:Journal Article

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