Please use this identifier to cite or link to this item: https://biore.bio.bg.ac.rs/handle/123456789/86
Title: Long-term dietary l-arginine supplementation increases endothelial nitric oxide synthase and vasoactive intestinal peptide immunoexpression in rat small intestine
Authors: Veličković, Ksenija 
Markelić, Milica 
Golić, Igor 
Otasevic, Vesna
Stancic, Ana
Jankovic, Aleksandra
Vucetic, Milica
Buzadzic, Biljana
Korać, Bato 
Korać, Aleksandra 
Keywords: Arginine;Nitric oxide synthase;Small intestine;Vasoactive intestinal peptide
Issue Date: 1-Jan-2014
Rank: M21
Project: White or/and brown: importance of adipose tissue in overall redox dependent metabolic control in physiological adaptations and metabolic disorders 
Journal: European Journal of Nutrition
Abstract: 
Background and aims: Nitric oxide (NO) and vasoactive intestinal polypeptide (VIP) are important intestinal neurotransmitters that coexist in the gut enteric nervous system and play an important role in intestinal physiology (e.g., absorption, motility, fluid secretion and smooth muscle relaxation). It is also known that cold exposure alters several aspects of gastrointestinal physiology and induces hyperphagia to meet increased metabolic demands, but there are no data regarding NO and VIP involvement in intestinal response during acclimation to cold. The objective of this study was to determine the influence of long-term l-arginine supplementation on the expression of the three isoforms of nitric oxide synthase (NOS) and VIP in small intestine of rats acclimated to room temperature or cold. Methods: Animals (six per group) acclimated to room temperature (22 ± 1 °C) and cold (4 ± 1°C), respectively, were treated with 2.25 % l-arginine, a substrate for NOSs, or with 0.01 % N ω-nitro-l-arginine methyl ester, an inhibitor of NOSs, for 45 days. The topographical distribution of VIP and NOSs expression in small intestine was studied by immunohistochemistry, and ImageJ software was used for semiquantitative densitometric analysis of their immunoexpression. Results: Long-term dietary l-arginine supplementation increases VIP and NOSs immunoexpression at room temperature while at cold increases the endothelial NOS, inducible NOS and VIP but decrease neuronal NOS in rat small intestine. Conclusion: Our results demonstrate that long-term dietary l-arginine supplementation modulates NOSs and VIP immunoexpression in rat small intestine with respect to ambient temperature, pointing out the eNOS as a predominant NOS isoform with an immunoexpression pattern similar to VIP. © 2013 Springer-Verlag Berlin Heidelberg.
URI: https://biore.bio.bg.ac.rs/handle/123456789/86
ISSN: 1436-6207
DOI: 10.1007/s00394-013-0585-8
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