Unveiling the Subterranean Symphony: A Comprehensive Study of Cave Fungi Revealed Through National Center for Biotechnology Sequences

Abstract

Caves can be regarded as extreme environments, and fungi are known as omnipresent and highly adaptable organisms that can easily colonize such environments. The primary objective of this study was to use the statistical analysis of sequences stored in the NCBI database, together with related metadata, to find and uncover statistically significant distribution patterns of fungi occupying different substrata inside the caves. The obtained list included a total of 1447 sequences corresponding to fungi isolated from various substrata within cave environments around the world, which corresponds to 445 fungal species, members of the 394 genera. Ascomycota was the most dominant phylum and Eurotiomycetes the dominant class of fungal dwellers in these environments. The highest species richness is detected for the genus Penicillium (57), followed by Aspergillus (51). On the other hand, the most frequently documented single species was Pseudogymnoascus destructans, isolated mostly from hibernating bats and guano, followed by Penicillium chrysogenum. Because caves have stable, nutrient-limited, low-competition microhabitats that support unusual or cryptic species, many new fungal taxa have been reported as well (such as Aspergillus, Apiotrichum, and Cephalotrichum species). Finally, cutting-edge molecular technologies and better sampling methods are revealing hitherto undiscovered fungal diversity in caves worldwide.