Effects of anoxia on³¹P NMR spectra of Phycomyces blakesleeanus during development

Abstract

The method of31P NMR spectroscopy was used to investigate the effects of anoxia on Phycomyces blakesleeanus mycelium during development. The greatest changes were recorded in the PPc, NADH, and α-ATP signals. Decrease of PPc signal intensity is due to chain length reduction and reduction in number of PPn molecules. Smaller decrease of β-ATP compared to α-ATP signal intensity can be attributed to maintenance of ATP concentration at the expense of PPn hydrolysis. Sensitivity to anoxia varies with the growth stage. It is greatest in 32-h and 44-h mycelium, in which PPn is used as an additional energy source, while the smallest effect was noted for 36-h fungi.