Please use this identifier to cite or link to this item: https://biore.bio.bg.ac.rs/handle/123456789/732
Title: Osmotic swelling activates a novel anionic current with VRAC-like properties in a cytoplasmic droplet membrane from phycomyces blakesleeanus sporangiophores
Authors: Križak, Strahinja
Nikolić, Ljiljana
Stanić, Marina
Žižić, Milan
Zakrzewska, Joanna
Živić, Miroslav 
Todorović, Nataša
Keywords: A-9-C;Filamentous fungi;Outward rectifier;Voltage-dependent inactivation;Whole-cell patch-clamp
Issue Date: 1-Apr-2015
Journal: Research in Microbiology
Abstract: 
© 2015 Institut Pasteur. We describe here whole-cell currents of droplets prepared from the apical region of growing Phycomyces blakesleeanus sporangiophores. Whole-cell current recordings revealed the osmotically activated, outwardly rectifying, fast inactivating instantaneous current (ORIC) with biophysical properties closely resembling volume-regulated anionic current (VRAC). ORIC is activated under conditions of osmotically induced swelling and shows strong selectivity for anions over cations. In addition, ORIC shows voltage and time-dependent inactivation at positive potentials and recovery from inactivation at negative potentials. ORIC is blocked by anthracene-9-carboxylic acid, an anion channel blocker, in a voltage-dependent manner. This is the first report of the presence of VRAC-like current in an organism outside the chordate lineage.
URI: https://biore.bio.bg.ac.rs/handle/123456789/732
ISSN: 0923-2508
DOI: 10.1016/j.resmic.2015.02.004
Appears in Collections:Journal Article

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