Please use this identifier to cite or link to this item: https://biore.bio.bg.ac.rs/handle/123456789/731
Title: <sup>51</sup>V NMR investigation of cell-associated vanadate species in Phycomyces blakesleeanus mycelium
Authors: Žižić, Milan
Miladinović, Zoran
Stanić, Marina
Hadžibrahimović, Mirzeta
Živić, Miroslav 
Zakrzewska, Joanna
Keywords: 51 V NMR;Fungi;Phycomyces blakesleeanus;Vanadate
Issue Date: 1-Jul-2016
Journal: Research in Microbiology
Abstract: 
© 2016 Institut Pasteur 51V NMR spectroscopy was used for detection and identification of cell-associated vanadate (V5+) species after exposure of Phycomyces blakesleeanus mycelium, in exponential phase of growth, to sodium orthovanadate. Complete disappearance of monomer and dimer signals and decreased intensity of the tetramer signal were observed about 40 min after treatment. Simultaneously, a signal at −532 ppm, with increasing intensity, was detected in spectra. The time-dependent rise in this signal was connected to a decrease in the extracellular monomer signal, indicating its transport into the cell. The signal at −532 ppm did not belong to any known simple oxido-vanadate species, nor to a complex with any of the components of experimental medium. This signal was the only one present in spectrum of the mycelium washed 35 min after treatment, and the only one observed in mycelium cultivated on vanadate-contained medium. Therefore, its appearance can be attributed to intracellular complexation, and may represent an important detoxification mechanism of the cell exposed to a physiologically relevant concentration of vanadate. Experiments (51V NMR and polarography) performed with Cd-pretreated mycelium (inhibitor of an enzyme responsible for V5+ reduction) and ferricyanide-preincubated mycelium excluded the possibility of V5+ tetramer's entry into the cell.
URI: https://biore.bio.bg.ac.rs/handle/123456789/731
ISSN: 0923-2508
DOI: 10.1016/j.resmic.2016.04.012
Appears in Collections:Journal Article

Files in This Item:
Show full item record

SCOPUSTM   
Citations

9
checked on Dec 20, 2024

Page view(s)

6
checked on Dec 23, 2024

Google ScholarTM

Check

Altmetric

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.