Please use this identifier to cite or link to this item: https://biore.bio.bg.ac.rs/handle/123456789/7194
Title: Short-Term l-arginine Treatment Mitigates Early Damage of Dermal Collagen Induced by Diabetes
Authors: Miler, Irena
Rabasovic, Mihailo D
Askrabic, Sonja
Stylianou, Andreas
Korać, Bato 
Korać, Aleksandra 
Keywords: AFM;Raman spectromicroscopy;dermal collagen I;diabetes;l-arginine;polarization-resolved SHG imaging
Issue Date: 21-Apr-2024
Rank: M22
Journal: Bioengineering (Basel, Switzerland)
Volume: 11
Issue: 4
Start page: 407
Abstract: 
Changes in the structural properties of the skin due to collagen alterations are an important factor in diabetic skin complications. Using a combination of photonic methods as an optic diagnostic tool, we investigated the structural alteration in rat dermal collagen I in diabetes, and after short-term l-arginine treatment. The multiplex approach shows that in the early phase of diabetes, collagen fibers are partially damaged, resulting in the heterogeneity of fibers, e.g., "patchy patterns" of highly ordered/disordered fibers, while l-arginine treatment counteracts to some extent the conformational changes in collagen-induced by diabetes and mitigates the damage. Raman spectroscopy shows intense collagen conformational changes via amides I and II in diabetes, suggesting that diabetes-induced structural changes in collagen originate predominantly from individual collagen molecules rather than supramolecular structures. There is a clear increase in the amounts of newly synthesized proline and hydroxyproline after treatment with l-arginine, reflecting the changed collagen content. This suggests that it might be useful for treating and stopping collagen damage early on in diabetic skin. Our results demonstrate that l-arginine attenuates the early collagen I alteration caused by diabetes and that it could be used to treat and prevent collagen damage in diabetic skin at a very early stage.
URI: https://biore.bio.bg.ac.rs/handle/123456789/7194
ISSN: 2306-5354
DOI: 10.3390/bioengineering11040407
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