Please use this identifier to cite or link to this item: https://biore.bio.bg.ac.rs/handle/123456789/7106
Title: A novel thermostable YtnP lactonase from Stenotrophomonas maltophilia inhibits Pseudomonas aeruginosa virulence in vitro and in vivo
Authors: Curcic, Jovana
Dinic, Miroslav
Novovic, Katarina
Vasiljevic, Zorica
Kojic, Milan
Jovčić, Branko 
Malesevic, Milka
Keywords: Antivirulence therapy;Lactonase;Pseudomonas aeruginosa
Issue Date: 28-Feb-2024
Rank: M21a
Publisher: Elsevier
Journal: International journal of biological macromolecules
Volume: 264
Issue: Pt 1
Start page: 130421
Abstract: 
Infections caused by multidrug-resistant pathogens are one of the biggest challenges facing the healthcare system today. Quorum quenching (QQ) enzymes have the potential to be used as innovative enzyme-based antivirulence therapeutics to combat infections caused by multidrug-resistant pathogens. The main objective of this research was to describe the novel YtnP lactonase derived from the clinical isolate Stenotrophomonas maltophilia and to investigate its antivirulence potential against multidrug-resistant Pseudomonas aeruginosa MMA83. YtnP lactonase, the QQ enzyme, belongs to the family of metallo-β-lactamases. The recombinant enzyme has several advantageous biotechnological properties, such as high thermostability, activity in a wide pH range, and no cytotoxic effect. High-performance liquid chromatography analysis revealed the activity of recombinant YtnP lactonase toward a wide range of N-acyl-homoserine lactones (AHLs), quorum sensing signaling molecules, with a higher preference for long-chain AHLs. Recombinant YtnP lactonase was shown to inhibit P. aeruginosa MMA83 biofilm formation, induce biofilm decomposition, and reduce extracellular virulence factors production. Moreover, the lifespan of MMA83-infected Caenorhabditis elegans was prolonged with YtnP lactonase treatment. YtnP lactonase showed synergistic inhibitory activity in combination with gentamicin and acted additively with meropenem against MMA83. The described properties make YtnP lactonase a promising therapeutic candidate for the development of next-generation antivirulence agents.
URI: https://biore.bio.bg.ac.rs/handle/123456789/7106
ISSN: 01418130
DOI: 10.1016/j.ijbiomac.2024.130421
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