Please use this identifier to cite or link to this item: https://biore.bio.bg.ac.rs/handle/123456789/7100
Title: Persicaria amphibia, an old traditional remedy and wild edible herb: in vitro evaluation of cytotoxicity and antimicrobial properties
Authors: Maksimović, Milica
Jovanović, Marina
Nikolić, Biljana 
Tomić, Nina
Tenji, Dina
Stević, Tatjana
Mitić-Ćulafić, Dragana 
Keywords: antimicrobial activity;diet-derived therapy;doxorubicin;Nrf2 gene expression;Persicaria amphibia;quorum sensing
Issue Date: 1-Jan-2023
Rank: M23
Publisher: Institut za botaniku i botaničku baštu "Jevremovac", Biološki fakultet
Journal: Botanica Serbica
Volume: 47
Issue: 1
Start page: 1
End page: 8
Abstract: 
Persicaria amphibia (subfam. Polygonoideae), an aquatic macrophyte rich in dietary polyphenolics, is used as a traditional remedy and culinary herb. Nevertheless, P. amphibia from the Balkan region has been insufficiently studied and underutilized. Here, the cytotoxicity and antimicrobial properties of the previously chemically characterised ethanol extract of P. amphibia aerial parts were tested. The following methods were carried out: the MTT assay, qRT-PCR, microdilution assay, Chromobacterium violaceum screening assay (monitoring of quorum sensing, QS) and the agar plating method (antifungal activity). The study was conducted to determine the cytotoxic effects of P. amphibia against lung cancer cells (A549) and its combination with cytostatic doxorubicin (Dox). A dose-dependent decrease in cell viability (up to 82% reduction) and additive interactions of the tested agents were noted. Both alone and combined with Dox, P. amphibia reduced the expression of Nrf2 (p < 0.05). In terms of antimicrobial effects, P. amphibia exhibited an antipathogenic effect since it disrupted QS communication, which was evident through the inhibition of violacein production of C. violaceum CV025. The antifungal screening revealed that P. amphibia induced significant growth inhibition of Aspergillus spp. (28.23%). Based on the obtained results, further examination of the potential use of P. amphibia in modern phytotherapy and diet-derived cancer chemoprevention is encouraged.
URI: https://biore.bio.bg.ac.rs/handle/123456789/7100
ISSN: 18212158
DOI: 10.2298/BOTSERB2301001M
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