Please use this identifier to cite or link to this item: https://biore.bio.bg.ac.rs/handle/123456789/684
Title: Isolation of presumptive plasma membranes from a characean alga
Authors: Anđus, Pavle 
Beljanski, Miloš
Keywords: adenosine triphosphatase activity;Chara;phosphotungstic acid-chromic acid;plasma membrane;two-phase polymer system
Issue Date: 1-Jan-1992
Journal: Plant Science
Abstract: 
The internodal cells of characean algae were frequently used as experimental models in studies of plant cell membrane control. However, these studies lacked a well defined in vitro system. Therefore, a method was introduced, based on membrane partitioning on an aqueous two-phase polymer system, by which the plasma membrane fraction from a characean alga (Chara gymnophylla) was isolated for the first time. The plasma membranes were identified on electron-micrographs by phosphotungstic acid-chromic acid (PTAC) selective staining. The specific adenosine triphosphatase (ATPase) activity in the plasma membrane fraction was 20 ± 4 nmol Pi/min per mg, and it was sensitive to the inhibitor of the plasma membrane ATPase, vanadate. Inhibitors of ATPase of other origin had a smaller influence (azide, nitrate), or showed no inhibition (oligomycin). While dependent on Mg2+, the plasma membrane ATPase showed a very low sensitivity to K+, suggesting that it is not a H+, K+ exchanging ATPase as inferred from studies on intact cells. © 1992.
URI: https://biore.bio.bg.ac.rs/handle/123456789/684
ISSN: 0168-9452
DOI: 10.1016/0168-9452(92)90219-C
Appears in Collections:Journal Article

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