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Title: | Isolation of presumptive plasma membranes from a characean alga | Authors: | Anđus, Pavle Beljanski, Miloš |
Keywords: | adenosine triphosphatase activity;Chara;phosphotungstic acid-chromic acid;plasma membrane;two-phase polymer system | Issue Date: | 1-Jan-1992 | Journal: | Plant Science | Abstract: | The internodal cells of characean algae were frequently used as experimental models in studies of plant cell membrane control. However, these studies lacked a well defined in vitro system. Therefore, a method was introduced, based on membrane partitioning on an aqueous two-phase polymer system, by which the plasma membrane fraction from a characean alga (Chara gymnophylla) was isolated for the first time. The plasma membranes were identified on electron-micrographs by phosphotungstic acid-chromic acid (PTAC) selective staining. The specific adenosine triphosphatase (ATPase) activity in the plasma membrane fraction was 20 ± 4 nmol Pi/min per mg, and it was sensitive to the inhibitor of the plasma membrane ATPase, vanadate. Inhibitors of ATPase of other origin had a smaller influence (azide, nitrate), or showed no inhibition (oligomycin). While dependent on Mg2+, the plasma membrane ATPase showed a very low sensitivity to K+, suggesting that it is not a H+, K+ exchanging ATPase as inferred from studies on intact cells. © 1992. |
URI: | https://biore.bio.bg.ac.rs/handle/123456789/684 | ISSN: | 0168-9452 | DOI: | 10.1016/0168-9452(92)90219-C |
Appears in Collections: | Journal Article |
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