Please use this identifier to cite or link to this item: https://biore.bio.bg.ac.rs/handle/123456789/6810
Title: Leptin deficiency impairs adipogenesis and browning response in mouse mesenchymal progenitors
Authors: Veličković, Ksenija 
Leija, Hilda Anaid Lugo
Kosic, Bojana
Sacks, Harold
Symonds, Michael E
Sottile, Virginie
Keywords: Adipogenesis;Browning;Leptin;Leptin receptor;Progenitors;Uncoupling protein 1 (UCP1)
Issue Date: Sep-2023
Rank: M21
Publisher: ScienceDirect
Project: EU‐CASCADE fellowship scheme funded by the EU's 7th FP
Biotechnology and Biological Sciences Research Council
Journal: European journal of cell biology
Volume: 102
Issue: 3
Start page: 151342
Abstract: 
Although phenotypically different, brown adipose tissue (BAT) and inguinal white adipose tissue (iWAT) are able to produce heat through non-shivering thermogenesis due to the presence of mitochondrial uncoupling protein 1 (UCP1). The appearance of thermogenically active beige adipocytes in iWAT is known as browning. Both brown and beige cells originate from mesenchymal stem cells (MSCs), and in culture conditions a browning response can be induced with hypothermia (i.e. 32 °C) during which nuclear leptin immunodetection was observed. The central role of leptin in regulating food intake and energy consumption is well recognised, but its importance in the browning process at the cellular level is unclear. Here, immunocytochemical analysis of MSC-derived adipocytes established nuclear localization of both leptin and leptin receptor suggesting an involvement of the leptin pathway in the browning response. In order to elucidate whether leptin modulates the expression of brown and beige adipocyte markers, BAT and iWAT samples from leptin-deficient (ob/ob) mice were analysed and exhibited reduced brown/beige marker expression compared to wild-type controls. When MSCs were isolated and differentiated into adipocytes, leptin deficiency was observed to induce a white phenotype, especially when incubated at 32 °C. These adaptations were accompanied with morphological signs of impaired adipogenic differentiation. Overall, our results indicate that leptin supports adipocyte browning and suggest a potential role for leptin in adipogenesis and browning.
URI: https://biore.bio.bg.ac.rs/handle/123456789/6810
ISSN: 01719335
DOI: 10.1016/j.ejcb.2023.151342
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