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Title: | Chemical Characterization, Antioxidant Activity, and Cytotoxity of Wild-Growing and In Vitro Cultivated Rindera umbellata (Waldst. and Kit.) Bunge | Authors: | Todorović, Slađana Perić, Marija Nikolić, Biljana Mandić, Boris Cvetković, Stefana Bogdanović, Milica Živković, Suzana |
Keywords: | biological activity;ex situ conservation;lithospermic acid B;pyrrolizidine alkaloids;rosmarinic acid | Issue Date: | 1-Mar-2023 | Rank: | M21 | Publisher: | Basel: MDPI AG | Journal: | Horticulturae | Volume: | 9 | Issue: | 3 | Start page: | 381 | Abstract: | The aim of this study was to comparatively analyze chemical composition and biological activity of wild- and in vitro grown Rindera umbellata. Explants were cultivated on 0.003–0.3 M sucrose, fructose, or glucose. HPLC-DAD for quantifying rosmarinic (RA) and lithospermic B (LAB) acids and GC-MS/FID for qualitative pyrrolizidine alkaloids (PAs) detection were used. Antioxidant activity (DPPH and ABTS assays) and cytotoxicity (MTT test) were monitored. Identified PAs were 7-angeloyl heliotridane, lindelofine, 7-angeloyl heliotridine, 7-angeloyl-9-(+)-trachelanthylheliotridine, punctanecine, and heliosupine, with higher variability reported in wild-growing samples. Total phenolic contents (TPCs) were comparable in wild-growing and in vitro samples, but total flavonoid (TFC) and RA levels were multifold higher in in vitro samples. Notably, high concentration of LAB was detected in wild-growing roots. Amounts of 0.3 M and 0.1 M of sucrose were optimal for TFC and RA production, while maximal antioxidant activity was monitored in plants grown on 0.3 M sucrose. The MTT test indicated colorectal HT-29 as more sensitive than A549 lung adenocarcinoma and normal MRC-5 cells, showing selective sensitivity to wild-growing and 0.3 M sucrose samples. In conclusion, PAs in vitro, as well as TPC, TFC, RA, and LAB in both growing conditions were detected for the first time in R. umbellata. |
URI: | https://biore.bio.bg.ac.rs/handle/123456789/6803 | ISSN: | 2311-7524 | DOI: | 10.3390/horticulturae9030381 |
Appears in Collections: | Journal Article |
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