Please use this identifier to cite or link to this item: https://biore.bio.bg.ac.rs/handle/123456789/6803
Title: Chemical Characterization, Antioxidant Activity, and Cytotoxity of Wild-Growing and In Vitro Cultivated Rindera umbellata (Waldst. and Kit.) Bunge
Authors: Todorović, Slađana
Perić, Marija
Nikolić, Biljana 
Mandić, Boris
Cvetković, Stefana 
Bogdanović, Milica
Živković, Suzana
Keywords: biological activity;ex situ conservation;lithospermic acid B;pyrrolizidine alkaloids;rosmarinic acid
Issue Date: 1-Mar-2023
Rank: M21
Publisher: Basel: MDPI AG
Journal: Horticulturae
Volume: 9
Issue: 3
Start page: 381
Abstract: 
The aim of this study was to comparatively analyze chemical composition and biological activity of wild- and in vitro grown Rindera umbellata. Explants were cultivated on 0.003–0.3 M sucrose, fructose, or glucose. HPLC-DAD for quantifying rosmarinic (RA) and lithospermic B (LAB) acids and GC-MS/FID for qualitative pyrrolizidine alkaloids (PAs) detection were used. Antioxidant activity (DPPH and ABTS assays) and cytotoxicity (MTT test) were monitored. Identified PAs were 7-angeloyl heliotridane, lindelofine, 7-angeloyl heliotridine, 7-angeloyl-9-(+)-trachelanthylheliotridine, punctanecine, and heliosupine, with higher variability reported in wild-growing samples. Total phenolic contents (TPCs) were comparable in wild-growing and in vitro samples, but total flavonoid (TFC) and RA levels were multifold higher in in vitro samples. Notably, high concentration of LAB was detected in wild-growing roots. Amounts of 0.3 M and 0.1 M of sucrose were optimal for TFC and RA production, while maximal antioxidant activity was monitored in plants grown on 0.3 M sucrose. The MTT test indicated colorectal HT-29 as more sensitive than A549 lung adenocarcinoma and normal MRC-5 cells, showing selective sensitivity to wild-growing and 0.3 M sucrose samples. In conclusion, PAs in vitro, as well as TPC, TFC, RA, and LAB in both growing conditions were detected for the first time in R. umbellata.
URI: https://biore.bio.bg.ac.rs/handle/123456789/6803
ISSN: 2311-7524
DOI: 10.3390/horticulturae9030381
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