Please use this identifier to cite or link to this item: https://biore.bio.bg.ac.rs/handle/123456789/6626
Title: Phenotypic and genetic properties of susceptible and multidrug-resistant Pseudomonas aeruginosa isolates in Southern Serbia
Authors: Milojković, Marko
Nenadović, Željka
Stanković, Slaviša 
Božić, Dragana D
Nedeljković, Nataša Stanković
Ćirković, Ivana
Petrović, Marija
Dimkić, Ivica 
Keywords: RAPD PCR profiling;clinical isolates;multidrug resistance;phenotypic characteristics
Issue Date: 1-Sep-2020
Rank: M22
Publisher: Institut za Medicinska Istrazivanja i Medicinu Rada
Journal: Arhiv za higijenu rada i toksikologiju
Volume: 71
Issue: 3
Start page: 231
End page: 250
Abstract: 
Drug resistance of Pseudomonas aeruginosa is a leading problem in hospital infections. The aim of this study was to determine the best molecular genetic discrimination method for Pseudomonas spp. isolates among 94 outpatients and inpatients and see their grouping by phenotype characteristics (biofilm formation, frequency of serotypes, pigmentation, production of different class of beta-lactamases, and susceptibility to different antibiotic classes) and genotype. The most common serotypes were P1, P6, and P11, while co-productions of pyoverdine and pyocyanin were observed in 70 % of isolates. A total of 77.66 % isolates were mostly weak and moderate biofilm producers. Isolates were susceptible to colistin (100 %), aztreonam (97.87 %), imipenem (91.49 %), doripenem (90.43 %), and meropenem (84.04 %). MICs values confirmed susceptibility to ceftazidime and cefepime and singled out meripenem as the most effective inhibitor. Most isolates were resistant to aminoglycosides and fluoroquinolones. Only two isolates produced ESBL, eight were carbapenemase producers, and five isolates produced MBLs. Twenty-nine isolates were multidrug-resistant; 82.8 % of which produced both pigments, 58.3 % were non-typeable, while the P6 and P11 serotypes were equally distributed (16.7 %). Thirteen MDR isolates were strong enzyme producers. RAPD PCR analysis using primer 272 proved the best at discriminatory fingerprinting for Pseudomonas isolates, as it allocated 12 clusters. A correlation between DNA patterns and antibiotic resistance, production of pigments, serotypes distribution, and biofilm formation was not observed, and only confirmed higher genetic heterogeneity among P. aeruginosa isolates, which suggests that other molecular methods are needed to reveal potential relations between genotypic patterns and phenotypic characteristics.
URI: https://biore.bio.bg.ac.rs/handle/123456789/6626
ISSN: 00041254
DOI: 10.2478/aiht-2020-71-3418
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