Please use this identifier to cite or link to this item: https://biore.bio.bg.ac.rs/handle/123456789/6447
Title: Could alder buckthorn (Frangula alnus Mill) be a source of chemotherapeutics effective against hepato- and colorectal carcinoma? An in vitro study
Authors: Vuletić, Stefana 
Bekić, Marina
Sergej Tomić,
Nikolić, Biljana 
Cvetković, Stefana 
Ganić, Tea 
Mitić-Ćulafić, Dragana 
Keywords: F. alnus;;Antioxidant;;Cytotoxicity;;Cell cycle;;Apoptosis;;Mitochondrial membrane potential;;Genotoxicity.
Issue Date: Nov-2023
Rank: M23
Publisher: Elsevier
Journal: Mutation Research/Genetic Toxicology and Environmental Mutagenesis
Start page: 503706
Abstract: 
Among numerous types of cancer, hepatocellular and colorectal carcinoma are important causes of mortality. Given the nature of these cancer types and their resistance, it is of great importance to find new chemotherapeutics and therapy targets, so plant products seem to be an excellent choice in such search. The main goal of this study was to investigate anticancer activity of Frangula alnus ethyl-acetate extract (FA) and its dominant constituent emodin (E) on hepatocellular and colorectal carcinoma cell lines, HepG2 and HCT116, as well as on normal MRC-5 fibroblasts. Cytotoxicity was investigated in MTT test and both FA and E showed strong reduction of cell viability in cancer cells. Flow cytometer analysis demonstrated that FA and E led to G1 phase arrest and slight accumulation of cells in the G2/M phase; additionally, annexinV-FITC/7AAD dying showed that FA and E decreased cell viability and triggered apoptosis in all cell lines. FA and E evidenced strong genotoxic potential in comet assay performed on all cell lines, while tests measuring antioxidative potential (DPPH and TBA) demonstrated strong effect of FA. It could be concluded that both FA and E have significant anticancer activity against hepatocellular and colorectal carcinoma cell lines HepG2 and HCT116, but notable selectivity was not observed.
URI: https://biore.bio.bg.ac.rs/handle/123456789/6447
DOI: 10.1016/j.mrgentox.2023.503706
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