Please use this identifier to cite or link to this item: https://biore.bio.bg.ac.rs/handle/123456789/6434
Title: 3D in vitro model of a functional epidermal permeability barrier from hESC and iPSC
Authors: Petrova, Anastasia
Celli, Anna
Jacquet, Laureen
Dafou, Dimitra
Crumrine, Debra
Hupe, Melanie
Arno, Matthew
Hobbs, Carl
Čvoro, Aleksandra 
Karagiannis, Panagiotis
Devito, Liani
Sun, Richard
Adame, Lillian C.
Vaughan, Robert
McGrath, John A.
Mauro, Theodora M.
Ilić, Duško
Issue Date: 6-May-2014
Rank: M21
Publisher: Elsevier
Journal: Stem Cell Reports
Volume: 2
Issue: 5
Start page: 675
End page: 689
Abstract: 
Cornification and epidermal barrier defects are associated with a number of clinically diverse skin disorders. However, a suitable in vitro model for studying normal barrier function and barrier defects is still lacking. Here, we demonstrate the generation of human epidermal equivalents (HEEs) from human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs). HEEs are structurally similar to native epidermis, with a functional permeability barrier. We exposed a pure population of hESC/iPSC-derived keratinocytes, whose transcriptome corresponds to the gene signature of normal primary human keratinocytes (NHKs), to a sequential high-to-low humidity environment in an air/liquid interface culture. The resulting HEEs had all of the cellular strata of the human epidermis, with skin barrier properties similar to those of normal skin. Such HEEs generated from disease-specific iPSCs will be an invaluable tool not only for dissecting molecular mechanisms that lead to epidermal barrier defects but also for drug development and screening.
URI: https://biore.bio.bg.ac.rs/handle/123456789/6434
DOI: 10.1016/j.stemcr.2014.03.009
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