Please use this identifier to cite or link to this item: https://biore.bio.bg.ac.rs/handle/123456789/6308
Title: Leaves micromorphology, chemical profile, and bioactivity of in vitro-propagated Nepeta cyrenaica (Lamiaceae)
Authors: Alimpić Aradski, Ana 
Oalđe Pavlović, Mariana 
Janošević, Dušica 
Todorović, Slađana
Gašić, Uroš
Mišić, Danijela
Pljevljakušić, Dejan
Šavikin, Katarina
Marin, Petar 
Giweli, Abdulhmid
Duletić-Laušević, Sonja 
Keywords: Nepeta cyrenaica;;Biological activities;;Chemical composition;;In vitro propagation;;Micromorphology.
Issue Date: Aug-2023
Rank: M22
Publisher: John Wiley & Sons Ltd.
Journal: Phytochemical Analysis
Volume: 34
Issue: 6
Start page: 661
End page: 679
Abstract: 
Introduction: The endemic species Nepeta cyrenaica Quézel & Zaffran, native to northeastern Libya, is valued as an important honey-bearing plant.

Objectives: This study was aimed to examine the micromorphology, phytochemistry, and bioactivity of in vitro-propagated N. cyrenaica for the first time.

Materials and methods: The leaf indumentum was examined using light and scanning electron microscopy and further characterised for histochemistry. The chemical composition of essential oil (EO) was performed using GC-MS analysis, while dichloromethane (DCM), methanol (ME), ethanol (ET), and aqueous (AQ) extracts were analysed using qualitative and quantitative LC/MS analyses. The antioxidant activities of EO and extracts were assessed using three parallel assays, while enzyme-inhibiting effects were evaluated against four enzymes.

Results: The leaves bear various types of glandular trichomes, with lipophilic secretion predominating. The main EO component of EO was 1,8-cineole. A considerable number of phenolics and iridoids were tentatively identified in the ME extract. Quantitative LC/MS analysis confirmed that ferulic acid, rosmarinic acid, and epigallocatechin gallate were present in the highest amount in the extracts, in which three iridoids were also quantified. Although the ME extract contained the highest amount of polyphenolics and iridoids, the DCM extract showed the best overall biological potential. Additionally, EO exerted the strongest acetylcholinesterase and tyrosinase inhibition.

Conclusion: This study demonstrated that the endemic N. cyrenaica can be efficiently grown under in vitro conditions, where it develops various glandular trichomes that are thought to secrete and/or accumulate bioactive compounds with valuable medicinal potential.
URI: https://biore.bio.bg.ac.rs/handle/123456789/6308
DOI: 10.1002/pca.3257
Appears in Collections:Journal Article

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